treated rats, showed severe inflammatory cells infiltration
An optimized medium for screening of L-asparaginase
production by Escherichia coli.
Keywords: Submerge fermentation, L-asparaginase
, enzyme activity, optimum condition.
(5,11,28) The use of L-asparaginase
has been reported infrequently in treatment of avian neoplasia.
was estimated by Nesslerization as described by Imada et al.
Actinomycetes are recognized as comparatively less explored source for L-asparaginase
and therefore act as candidates for the production of L-asparaginase
Additionally, there was no significant difference in the number of thromboses related to L-asparaginase
that occurred in one patient in pediatric protocol 3% and 2 patients in adults protocol 3.7% in adults group (P = 0.091).
We know L-asparaginase
is very important to treat patients with ALL, and we also know the toxicities related to this drug.
(Elspar, Lundbeck, Deerfield, IL, USA) and L-asparagine (Sigma-Aldrich, St.
Unlike normal cells, the malignant cells require large amount of L-asparagine for protein synthesis and cell division, as L-asparaginase
catalyzes the deamination of L-asparagine into L-aspartic acid and ammonia, hence L-asparagine levels in the cancer cells decrease drastically, thereby it leads to the decrease in protein synthesis and cell division in tumor cells.
Growing colonies on the plate of L-asparaginase
in primary screening were screened for L-asparaginase
activity by rapid plate assay method using the modified Czapek Dox medium.
The other chemotherapy agents with which our patient had been treated (cytarabine, vincristine, L-asparaginase
, daunorubicin, and methotrexate) have no reported links to AVN.