Fucoidans are complex sulfated polysaccharides consisting mainly of sulfated L-fucose and variable proportions of galactose, mannose, xylose, glucose, rhamnose and uronic acids (Usov & Bilan, 2009).
These polysaccharides usually contain large proportions of L-fucose and sulfate, together with minor amounts of other sugars like xylose, galactose, mannose and glucuronic acid (Ponce et al, 2003).
is a rare monosaccharide with limited natural availability and a precursor of complex carbohydrates, as Human Milk Oligosaccharides (HMOs).
glutinins produce a heteropolysaccharide composed of L-fucose
and Dgalactosein an approximate molar ratio of 1:1.
Also, negative reaction for urease and L-fucose
assimilation differentiated the strain from S.
The specificity of lectin binding was tested by addition of free L-fucose
to the AAL solution before addition to the microtiter plate.
The ratio of L-rhamnose, L-fucose
, L-galactose, and D-galacturonic acid was 2.
Specifically, the project targets L-fucose
and fucosylated oligosaccharides that are very difficult to produce through extraction or chemical synthesis.
5 M solutions of [alpha]-mannose, D-glucose, L-fucose, D-galactose, N-acetyl-glucosamine or N-acetyl-galactosamine which are known competitors of the lectins LCA, Con-A, LTA, BS-1, GSL II and DBA respectively.
typus this sugar has a great importance in the fertilization process because in spite of its small concentration (similar to that of L-fucose, D-galactose and a-GalNAc) in the intact oocyte and isolated envelopes it was the strongest inhibitor of fertilization together with glucose and mannose among the seven sugars tested in this study.