The detection wavelength for quantitative analysis was selected according to the maximum adsorption wavelengths of D-mannose, L-rhamnose monohydrate, glucose, galactose, and xylose at 250 nm, as shown in Figure 1.
As shown from Figures 1 and 2, there were D-mannose, L-rhamnose, glucose, and xylose in SLGI.
, and D-glucose are hereafter referred to as mannose, rhamnose, and glucose in the text.
It was extracted with EtOAc and the residue recovered from the organic phase was found to be dibenzofruran while the aqueous phase was concentrated and purified by preparative TLC using the solvent system (EtOAc-MeOH-H2O-HOAc; 4: 2: 2: 2) and identified as D-glucose and L-rhamnose by comparative TLC of the hydrolyzed sugars with the authentic samples of glucose and rhamnose and their optical rotation values [[alpha]]D20 + 50.5Adegand [[alpha]]D23 +7.7Adeg.
The two sugar moieties were finally identified as D-glucose and L-rhamnose due to acidic hydrolysis of compound 1 followed by comparative TLC of the hydrolyzed sugars with the authentic samples of glucose and rhamnose and their optical rotation values [[alpha]]D20 + 50.5Adeg(c, 10 in H2O)  and [[alpha]]D23 +7.7Adeg (c, 0.2 in H2O) .
Monosaccharides such as mannitol and L-rhamnose pass through the transcellular routes of aqueous pores, reflecting the degree of absorption of small molecules (0.65 nm).
Intestinal permeability to lactulose/ L-rhamnose in children with celiac disease and other gastrointestinal disorders [Letter].
Both the sugars were identified as D- galactose and L-rhamnose through their optical rotation values [a]20 +78.5 (c 1 in H2O)  and [a]23 +7.7 (c 0.2 in H2O)  and comparison of the retention time of their trimethylsilyl (TMS) ethers with those of the standards in gas chromatography (GC).
The aqueous phase was concentrated under reduced pressure and purified on preparative thin layer chromatography using solvent system (EtOAc-MeOH-H2O-HOAc; 4:2:2:2) and identified as D-galactose and L-rhamnose by the sign of their optical rotation ([a]D20 +78.5 ) and ([a]D20 +7.7) respectively.