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The imbalance of trout's bacterial flora and the extinction of proteolytic bacteria from bacterial communities of the digestive tract of exposed fish were determined after treatment to the highest concentration (0.25 portion of the LC50 of Cu+ Zn mixture).
Values of LC50 and EC50 published by several authors in different species of marine invertebrates and in our study are shown in Table 2.
Keywords: LC50, lethal concentration, metal toxicity, Catla catla, Labeo rohita, Cirrhina mrigala
Didesniu vienadieniu lervu kvepavimo daznio pokyciu nenustatyta veikiant 0,125 LC50 Cu (0,045 mg/l) bei Cu ir Zn misiniu (0,045 + 0,12 mg/l).
Estimacion Valores de LC y Limites de Confianza Concentracion Limites de confianza (95%) Exposicion Inferior Superior LC1 0,036 0,000 0,120 LC5 0,080 0,001 0,198 LC10 0,122 0,003 0,262 LC15 0,162 0,008 0,319 LC50 0,546 0,246 1,134 LC85 1,838 0,953 33,405 LC90 2,449 1,164 83,842 LC95 3,747 1,542 333,099 LC99 8,322 2,546 4,238,567 Chi-Cuadrado (0,05): 9,488 Tabla 2.
Methyl parathion is one of the more toxic active ingredients used on the SHP, with a 48 hour LC50 value of 0.14 [micro]g/L for the aquatic invertebrate D.
The differences in the LC50 value for both the species were statistically significant (p<0.001, F-test, Table 2).
Each group was transferred to a plastic vial and treated with a toxic concentration corresponding to the LC50. Dead adults were counted twice a day, and the treatment was stopped when about 90% of the flies were dead.
The lethal concentration LC50 of each essential oil was also calculated by probit analysis.
Compounds###Linear regressive equation(y=a+bx)###Correlation coefficient(r)###LC50(mg/L)###95% confidence interval of LC50
Fractions 1, 2, 6 and 7 caused LC50 equaled 463.6 and 296.4 ug/ml; 321 and 166.8 ug/ml; 403 and 292 ug/ml and 1100 and 927.7 ug/ml after 24 and 48 hours exposure, respectively.
Phytochemical screening was done to support the results of the following tests: toxicity test by calculating LC50 using brine shrimp lethality test; antioxidant screening using thin-layer chromatography; and purgative test by anthelmintic assay using Eudrilus eugeniae as test organisms.
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