ligase


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ligase

[′lī‚gās]
(biochemistry)
An enzyme that catalyzes the union of two molecules, involving the participation of a nucleoside triphosphate which is converted to a nucleoside diphosphate or monophosphate. Also known as synthetase.
References in periodicals archive ?
These results indicated that the N-terminal portion of Hbo1 was required for E3 ligase activity toward ER[alpha] LBD.
Glutamate-cysteine ligase modifier subunit: mouse Gclm gene structure and regulation by agents that cause oxidative stress.
reported that the overexpression of the ubiquitin E3 ligase Parkin, which was found reduced in human AD brain, greatly decreased the levels of intracellular A[beta]-42 in neurons [57].
The PCR step first amplifies all epialleles present in a sample, and the ligase step, exploiting its sensitivity to mismatches up to 10 bases from the 3; ligating end (14, 25, 32), is used to extract the proportion of the epiallele patterns.
The pair quickly discovered they were both looking at the role of Ligase 3 in cancer and decided to collaborate.
If (but only if) the 3' end of primer 1 anneals to the template, an enzyme known as DNA ligase catalyzes the covalent joining of the 3'-OH of primer 1 to the 5' phosphate of primer 2.
MARCH5 has been predicted to contain four transmembrane domains, [38, 39] while MARCH6, which was identified as a mammalian homolog of the yeast E3 ligase Doa10, has up to 14 putative transmembrane domains [40,41].
T4 DNA ligase (1 uL, NEB), 10 x ligase buffer (1 uL, NEB) were added and the final volume made up to 10 uL with nuclease-free water.
The fruit flies' reduced distaste for camphor occurred through a mechanism that involves the degradation of the TRPL protein by an enzyme called E3 ubiquitin ligase, or Ube3a, which targets specific protein substrates for degradation.
Then, insert DNA is ligated into plasmid vector by DNA ligase.