According to the study, the maximum toxicity by pesticide pyriproxyfen was observed to nodule numbers in pea, root and shoot dry biomass, shoot nitrogen and root phosphorus in greengram, leghaemoglobin
, seed protein and chlorophyll content in chickpea.
Dilworth, "Control of leghaemoglobin
synthesis in snake beans," Biochemical Journal, vol.
Nodulation and nitrogen fixation depend on the capability of rhizobia for effective (nitrogen fixing) nodule formation, which was formed by synthesis of leghaemoglobin
(Subba Rao, 2000) that increased nitrogenase activity for fixing higher amount of nitrogen.
A nodulated legume has an increased need for iron compared to a nonnodulated plant  since this metal is a constituent of key proteins such as nitrogenase and leghaemoglobin
. Nitrogenase is made up of two proteins; both are rich in iron and essential for activity.
Nodules were removed, counted and sliced cross-sectionally for assessment for potential BNF using the leghaemoglobin
pigment indicator Thereafter, each sample's nodules gravimetric moisture content and eventual dry nodule dry weight was determined.
N2 fixation (C2H2-reducing activity) and leghaemoglobin
content during nitrate and water stress-induced senescence of Medicago sativa root nodules.
1987.5' Analysis of the soybean leghaemoglobin
lbc3 gene: Regulatory elements required for promoter activity and organ specificity.
We know it is an effective siderophore and that it can make increased amounts of iron available from the soil to the rhizobia-plant symbiosis, which helps to make the heme protein in leghaemoglobin
. This is one quarter of a human haemoglobin molecule and performs the same function.
Increase in the protease activity of nodules was paralleled by a decline in leghaemoglobin
content beginning 31 DAE (Fig.
(1986), Abd-Alla (1992), and Abd-Alla and Abdel Wahab (1995a), studying the effect of water stress on leghaemoglobin
metabolism, found that water stress lowered the content of leghaemoglobin
and decreased respiration and ATP production.