Lineweaver-Burk equation


Also found in: Medical.

Lineweaver-Burk equation

[¦līn‚wēv·ər ′bərk i‚kwā·zhən]
(biochemistry)
A double reciprocal form of the Michaelis-Menten equation, written as 1/ V0= Km / Vmax[S ] + 1/ Vmax, that yields a straight-line plot for reactions obeying basic Michaelis-Menten kinetics. It provides a more accurate estimate of Vmaxand is helpful in analyzing enzyme inhibition.
References in periodicals archive ?
For the dynamic fluorescence quenching, its binding constant (KA) and the number of binding site (n) could be obtained by using the following Lineweaver-Burk equation:
The Lineweaver-Burk equation canbe derived from the Michaelis-Menten equation:
These experimental results are similar to the maximum specific growth rate [[mu].sub.max] ([h.sup.-1]) calculated by the Lineweaver-Burk equation. A significant influence of the initial substrate concentration on the maximum specific growth of fermentations A and B was not observed.
The kinetic parameters - maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) for the purified protease was estimated from the Lineweaver-Burk equation plot26.