After 24 hours, the medium was aspirated with Thermo Scientific Versette Automated Liquid Handler, and luciferase
activity was measured in each well with the Dual Luciferase
Assay Reporter System (Promega).
Researchers are now able to easily insert luciferase
genes into the cells of interest, administer them to animal models, and immediately identify not only the organs that they populate but also their viability, proliferation, and any changes in biodistribution that might occur over time.
for bioluminescence reporter and imaging applications.
Many proteins are used as reporter proteins , for example chloramphenicol acetyltransferase (CAT) , [beta] galactosidase and luciferase
. Firefly luciferases
are abundantly utilized as gene reporter and catalyze a bioluminescense reaction which are directly detected.
These creatures all owe their glow to an enzyme called luciferase
These cells contain luminous materials: luciferin and either luciferase
or a luciferin-binding photoprotein.
In addition, the expressed enzyme and the gene for the bioluminescent luciferase
class of enzymes have considerable potential as labels for nucleic acid assays.
"A real revolution has come from our ability to look at how genes work," says Wood, who first basked in the glow of scientific luminosity in 1986 when he and co-workers made the world's first glow-in-the-dark tobacco plants by splicing into the plants a luciferase
gene from a firefly.
The molecular weights of luciferases
A, B, and C were estimated by gel filtration on the same Superdex 200 Prep column that was used in Step 6 to purify the luciferases
; 0.005% LCC was added to the buffer to minimize adsorption onto the column.
Significance of the existence of two different luciferases
Promoter activity was assessed by measuring luciferase
Friedrich and Greenberg  reported that growth on carbon source other than glycerol such as glucose elicited catabolite repression of luciferase
activity, hence the lag phase observed.