After 24 hours, the medium was aspirated with Thermo Scientific Versette Automated Liquid Handler, and
luciferase activity was measured in each well with the Dual
Luciferase Assay Reporter System (Promega).
Researchers are now able to easily insert
luciferase genes into the cells of interest, administer them to animal models, and immediately identify not only the organs that they populate but also their viability, proliferation, and any changes in biodistribution that might occur over time.
Red-emitting
luciferases for bioluminescence reporter and imaging applications.
Many proteins are used as reporter proteins , for example chloramphenicol acetyltransferase (CAT) , [beta] galactosidase and
luciferase. Firefly
luciferases are abundantly utilized as gene reporter and catalyze a bioluminescense reaction which are directly detected.
These creatures all owe their glow to an enzyme called
luciferase.
These cells contain luminous materials: luciferin and either
luciferase or a luciferin-binding photoprotein.
In addition, the expressed enzyme and the gene for the bioluminescent
luciferase class of enzymes have considerable potential as labels for nucleic acid assays.
"A real revolution has come from our ability to look at how genes work," says Wood, who first basked in the glow of scientific luminosity in 1986 when he and co-workers made the world's first glow-in-the-dark tobacco plants by splicing into the plants a
luciferase gene from a firefly.
The molecular weights of
luciferases A, B, and C were estimated by gel filtration on the same Superdex 200 Prep column that was used in Step 6 to purify the
luciferases; 0.005% LCC was added to the buffer to minimize adsorption onto the column.
Significance of the existence of two different
luciferasesPromoter activity was assessed by measuring
luciferase activities.
Friedrich and Greenberg [38] reported that growth on carbon source other than glycerol such as glucose elicited catabolite repression of
luciferase activity, hence the lag phase observed.
