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(chemical engineering)
Rapid freezing of a material, especially biological specimens for preservation, at a very low temperature followed by rapid dehydration by sublimation in a high vacuum.



freeze-drying, the drying of biological objects in a frozen state under vacuum, the water being removed by sublimation of the ice (that is, conversion to steam, bypassing the liquid phase).

Lyophilization makes it possible to obtain dry tissues, medical preparations, and food products without a loss of structural integrity and biological activity. Most proteins are not denatured by the process and can be stored for a long time afterward under moderate cold (about 0°C). Freeze-dried tissues and preparations regain their initial properties when wetted. The process is used when there is a need for the long-term storage and preservation of various products of biological origin; to obtain dry donor plasma and dry sera and vaccines; for organ and tissue transplants; and in the pharmaceuticals and food industries. In the life-support systems of spaceships, it may be used as a means of regenerating water from moisture-containing materials.


Primenenie zamorazhivaniia-vysushivaniia ν biologii. Edited by R. Harris. Moscow, 1956. (Translated from English.)
References in periodicals archive ?
Lyophilization is now used in the food and pharmaceutical industries to preserve sensitive materials such as protein drugs, vaccines, synthetic tissues, and probiotic cultures--among many others.
The SA25 will be installed for liquid GMP manufacturing by the fourth quarter of 2017, with added lyophilization capabilities beginning in 2018.
Founded in 1997, LSNE is a market leader providing a wide range of development and manufacturing services to the pharmaceutical, biotechnology and medical device industries, specializing in aseptic fill/finish and lyophilization services.
This report provides both, qualitative and quantitative analyses of the market for lyophilization equipment.
Since World War II (WWII) lyophilization has been used to stabilize living cells and fungal isolates.
To overcome this issue, Actelion Pharmaceuticals adopted the fast lyophilization (LyoSpeed) methodology developed by Genevac, and implemented this using the Genevac HT-12 centrifugal evaporation systems.
Therefore, the extra steps of previous lyophilization and PVP addition during the maceration might ensure efficiency and reliability, improving the amount of DNA purified as well as reducing problems with the co-isolation of undesirable constituents, such as polysaccharides and polyphenols.
Many biotechnology products require lyophilization, a process of freeze-drying sterile solutions to permit a shelf stable dosage form.
The non-profit centre, also known as Regen Med, is finalizing a new business plan and contract that will bring the lyophilization method and some advanced tissue cutting techniques to Thunder Bay.
The process of lyophilization, or freeze drying, is used to dehydrate samples in order to preserve them for transportation or future study or to prepare them for analysis.
This compilation of new research on one of the most important and frustrating aspects of research in this field has been completely updated and features notes for each protocol, methods for conservation and sustainable use of resources, methods for lyophilization of proteins, and new and new techniques for preserving biological materials.