Matrix metalloproteinases (MMPs) are special class of proteases that are categorized in five groups as per their substrate specificity: collagenases (MMP-1, -8 and -13), gelatinases (MMP-2
and -9), stromelysins (MMP-3, -10, and -11), matrilysins (MMP-7) and membrane-type (MMP-14 to -17, -24 and -25).
Gelatin zymography assay was performed to evaluate the activity of MMP-2
and MMP-9 as previously described (12).
Research showed that test mice, vaccinated with peptides containing specific sequences from MMP-2
and MMP-9 and subsequently challenged with melanoma cancer cells, had an average reduction in tumour volume by about 76% compared to unvaccinated controls.
and MMP-9 are members of gelatinase group and are able to degrade gelatin and collagen (2).
We investigated the differences of MMP-2
expression between LV and RV in response to nandrolone decanoate (ND), swimming training (ST), and combined ND and ST (NS) in mice, based on their structural, functional, and biochemical characteristics.
Twenty-four MMPs have been identified, including collagenases (MMP-1, 8, 13, and 18), gelatinases (MMP-2
and 9), stromelysins (MMP-3 and 10), matrilisins (MMP-7 and 26), and membrane-type MMPs (MMP-14, 15, 16, 17, 24, and 25), among other types.
The Membrane Penetration Effect of FITC-iCREKA after Cleavage by MMP-2
. (1) Examination of the binding of FITC-iCREKA to U87 glioma cells after MMP-2
White, "The direct inhibition of MMP-2
and MMP-9 by an enzyme alginogel: A possible mechanism of healing support for venous leg ulcers," Journal of Wound Care, vol.
Depending on the substrate specificity and structure, MMPs are divided into several subgroups: collagenases (e.g., MMP-1), gelatinases (e.g., MMP-2
and MMP-9), stromelysins (e.g., MMP-3 and MMP-10), matrilysins (e.g., MMP-7 and MMP-26), and membrane-type matrix metalloproteinase-1 (MT1-MMP).
Second group comprises gelatinases: MMP-2
(gelatinase A) and MMP-9 (gelatinase B).
The vascular reactivity and time-course changes of collagen type I, MMP-2
, membrane type 1-MMP (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) protein expression in mesenteric arteries were evaluated.
Primary polyclonal antibodies of rabbit anti-rabbit collagen type I (1:20, Bioss Bio., China), rabbit anti-rabbit MMP-2
(1:50, Boster Bio., China), and rabbit anti-rabbit MMP-9 (1: 20, Bioss Bio., China) were applied overnight at 4[degrees]C.