Ascertainment and identification are done by measurement of mass to charge ratio
and abundance of gas phase ions within the sample.
The RF voltage on the trap electrodes is ramped, causing ions to eject according to their mass to charge ratio
. Ions and ion fragments impact a faraday detector and are amplified and processed.
M/Z NHS (mean [+ or -] SD) OS (mean [+ or -] SD) 3954.00 Da 1.75 [+ or -] 0.35 -0.78 [+ or -] 0.08 6438.00 Da 0.45 [+ or -] 0.21 0.88 [+ or -] 0.13 M/Z OC (mean [+ or -] SD) P value VI (score) 3954.00 Da 1.47 [+ or -] 0.35 0.001 100 6438.00 Da 5.78 [+ or -] 1.12 0.001 100 M/Z: mass to charge ratio
; NHS: normal human serum; OS: osteosarcoma; OC: osteochondroma; VI: variable importance.