The carcinogen DMBA, biochemical compounds - reduced glutathione (GSH), reduced nicotinamide adenine dinucleotide (NADH), colchicine, giemsa, 1,1',3,3' tetramethoxy propane, bovine serum albumin, and May-Grunwald stain
were procured from Sigma-Aldrich Pvt Ltd, Bengaluru, India.
The devices were removed from the medium at 24h, 48h and 72h after seeding, washed and fixed with 0.5% gluteraldehyde for 1h and stained using May-grunwald stain. The devices were opened using a pair of scissors and then viewed under light microscope (Lieca MPS30, Switzerland), and the cell attachment was assessed.
The devices were taken out and rinsed thoroughly with buffer and after fixation with 0.2% gluteraldehyde for 1h at room temperature, were stained using May-Grunwald stain. Samples were viewed under Light Microscope (Lieca MPS30, Switzerland) to analyze leukocyte adhesion.