Presence of time hierarchy makes possible the saturated response of the output to the WIP in the form of the Karmarkar clearing function (in fact the Michaelis-Menten equation
), whereas the side buffer leakage secures finiteness of the steady-state inventory level.
The relationship between the reciprocal of the response current ([i.sup.-1]) and the reciprocal of the lactose concentration ([C.sup.-1]) was obtained according to the Lineweaver-Burk form of the Michaelis-Menten equation
(Figure 9) .
Kinetics properties of L.plantarum uricase were determined using Michaelis-Menten equation
and Lineweaver-Burk plot, where:
The Km and Vmax were calculated using Michaelis-Menten equation
at pH 4 and 50[degrees]C.
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation
The kinetic parameters of [alpha]-glucosidase inhibitory protein were determined by using LineweaverBurk plots (22) and Michaelis-Menten equation
and its reciprocal.
Two different substrate concentrations were chosen to explain enzyme kinetics, using the Michaelis-Menten equation
. With two values for initial velocities and substrate concentrations, [V.sub.max] and [K.sub.m] values of the reactions could be established (Figure 8).
The Lineweaver-Burk equation canbe derived from the Michaelis-Menten equation
The curve which expresses the relationship between substrate concentration and initial velocity has the same shape for most enzymes, a rectangular hyperbola, whose algebraic expression is given by Michaelis-Menten equation
(1), which describes the relationship between initial velocity [V.sub.o] and substrate concentration, S :
Kinetic parameters were determined by fitting the data to the Michaelis-Menten equation
using GraFit .
As well known, the electrical response of an amperometric biosensor is well described by Michaelis-Menten equation
The model used for ChiA from Serratia marcescens (Equation 1) fit satisfactorily to the experimental data with colloidal chitin, while for 4-MUF kinetics the Michaelis-Menten equation
for substrate inhibition fit better (Figure 6B).