KEY WORDS: BDE-47, BDE metabolites, CYP2B6, human liver microsomes
, hydroxylated metabolites, PBDE metabolism.
450] contents were expressed as [eta]mol cytochrome/mg microsomal protein, glutathione S-transeferase activity was expressed as units/mg protein, NADPH cytochrome C-reductase activity was expressed as [eta]mol cytochrome C-reductase/mg protein/min, amidopyrine N-demethylase in brain microsomes
was expressed as [micro]mol/min x kg brain sample, aniline-4-hydroxylase was expressed as [micro]mol/min/mg protein, and thiobarbituric acid reactive substances (TBARS) were expressed as [micro]mol TBARS/g tissue.
Inhibition of deiodinase activity by TBMEHP in rat liver microsomes
Celsis IVT has "purpose-pooled" InVitroCYPs to meet a variety of research needs by using a proprietary technology in which microsome
activity levels are optimized across nine key CYP substrates for their respective assay applications.
Lipid peroxidation marked by MDA level, particular dramatic ROS eruption, was found as free microsomes
were incubated with ethanol (100 mM) for 1 h.
This plate-incorporation version of the Salmonellal mammalian microsome
mutagenicity assay became a standard that is still in use today.
were prepared through ultracentrifugation which was developed in our laboratory (Li et al.
The effects of Danshen and its active components (tanshinone I, tanshinone IIA, dihydrotanshinone and cryptotanshinone) on CYP2D6 activity was investigated by measuring the metabolism of a model CYP2D6 probe substrate, dextromethorphan to dextrorphan in human pooled liver microsomes
Table 1 Inhibition of nine cytochrome P450 (CYP)-catalyzed activities by machilin A (MA) in pooled human liver microsomes
In in vitro studies, CsA has induced lipid peroxidation in rat kidney and liver microsomes
A previous study had proposed a 3 x 4 scheme of metabolic pathways of DEET in rat liver microsomes
(Constantino & Iley, 1999).
SRL (100 [micro]g/L) was incubated with individually prepared human liver microsomes
(HLM) (n = 31) (donor characteristics are given in Table 1 in the Data Supplement that accompanies the online version of this report at http://www.