The assay contained 100 [micro]g mitochondrial protein
, 10 mM HEPES (pH 8.0), 100 mM Na[N.sub.3], 100 [micro]M N[O.sub.4]Na, 90 mM KCl, 3 mM MgS[O.sub.4]; the ATP regenerating system consisted of 5 mM phosphoenolpyruvate, 2 mM ATP, 0.03% zwittergent, 50units/mL pyruvate kinase, and 30units/mL lactate dehydrogenase.
In rats fed a ketogenic diet (Bio-Serv F3666) for 22 days, mitochondrial density (determined by electron microscopy) in the hippocampus increased in conjunction with increased transcription of 39 of the 42 mitochondrial proteins
Consistent with previous studies , the proteins from the mitochondrion (88,405 proteins from 6119 species) and chloroplast (80,807 proteins form 935 species) sets have relatively low disorder contents compared to the proteins encoded in nuclear genes of eukaryotic organisms, for example, the mitochondrial protein
set has a considerably lower disorder content of 8.6% at protein level.
Hoffman et al., "Alpha-S-ynuclein binds to TOM20 and inhibits mitochondrial protein
import in Parkinson's disease," Science Translational Medicine, vol.
In this review, the expressed sequence tags of some nuclear transcripts encoding mitochondrial proteins
were selected from the Litopenaeus vannamei transcriptome database (Ghaffari et al.
In spite of being helpful in assessing mitochondrial functional integrity, maximal capacity of oxidative phosphorilation and import of mitochondrial proteins
, this method has several limitations:
A recent study demonstrated that ethanol induces mitochondrial protein
hyperacetylation (excessive modification by acetylation of the lysine residues of a protein) in the kidney, which might interfere with the function of some mitochondrial proteins
involved in alcohol metabolism or defense against oxidative stress (e.g., superoxide dismutase 2, aldehyde dehydrogenase 2, gluthatione peroxidase).
Recently, proteomics analyses in several studies have suggested that changes in some mitochondrial proteins
are involved in cardio-protection [2-5].
We studied the effect of a high-glucose environment on the expression of the key transcriptional coactivator of mitochondrial proteins
, PGC1[alpha], in human renal proximal tubular cells after treatment with 5 mM (low) or 30 mM (high) D-glucose for 24 h.
Cataloging DNA in the microbe turns up none of the known genes for mitochondrial proteins
. Stealing genetic material from bacteria--which also survive without mitochondria--allowed the microbe to do without them, researchers report in the May 23 Current Biology.
Mitochondria from the calluses were isolated and the total mitochondrial proteins
SIRT3 acts as a primary mitochondrial deacetylase and targets multiple of mitochondrial proteins
. These proteins are acetylated in TCA cycle and [sz]-oxidation pathways implicated in carbon conversion and energy metabolism within mitochondria.