EGFR Mutation Testing
on Cytological and Histological Samples in Non-Small Cell Lung Cancer: a Polish, Single Institution Study and Systematic Review of European Incidence.
M2 EQUITYBITES-August 2, 2019-Sysmex awarded Japanese manufacturing and marketing approval for first blood-based RAS mutation testing
for colorectal cancer
M2 PHARMA-August 2, 2019-Sysmex awarded Japanese manufacturing and marketing approval for first blood-based RAS mutation testing
for colorectal cancer
An alternate strategy for EGFR and ALK testing was therefore developed using material from the liquid-based cytology sample for EGFR mutation testing
by next-generation sequencing (NGS) and the ThinPrep slide for fluorescence in situ hybridization (FISH) testing for ALK gene rearrangements.
Patients who are negative by the therascreen test using the liquid biopsy should undergo tumor biopsy for PIK3CA mutation testing
. "Piqray is the first PI3K inhibitor to demonstrate a clinically meaningful benefit in treating patients with this type of breast cancer.
Formalin-fixed paraffin-embedded (FFPE)  tissue is typically used as a source of DNA for somatic mutation testing
. FFPE tissue sections are processed by deparaffinization, digestion, and DNA extraction.
BRCA mutation testing
for breast and ovarian cancer susceptibility is advisable, especially if there is any family history of cancers.
For the index patient, mutation testing
included RET gene exons 8, 10, 11, 13, 14, 15, and 16 and their flanking regions using PCR and direct sequencing of DNA.
The diagnostic platform Idylla seems to be an interesting alternative for the time-consuming and costly next-generation sequencing (NGS) techniques used for point mutation testing
. The Idylla NRAS Mutation Test, performed on the Biocartis Idylla system, is an in vitro diagnostic tool for the qualitative assessment of 18 NRAS mutations in codons 12, 13, 59, 61, 117, and 146 .
Data were collected based on patient sex, date of birth, age at diagnosis, stage at diagnosis, method of tumour sample collection, whether the sample was sent for EGFR mutation testing
, and the results of testing.
Amos et al., "Standards and guidelines for CFTR mutation testing
," Genetics in Medicine, vol.