The fraction V3 (2.8 g) obtained from the main column with n-hexane
: ethyl acetate (6: 4) was further subjected to silica gel column to give compound 2 (19 mg) and 8 (17 mg) when eluted with n-hexane
: ethyl acetate (5: 5).
Initially, the PCDDs from spiked grain samples were extracted through 1:1 n-hexane
The reaction mixture (2 mL) consisted of 1 mL of test sample (methanol extract, water, ethyl acetate, chloroform, and n-hexane
fractions) and 1 mL of 10% RBC suspension; instead of test sample only saline was added to the control test tube.
The extract was fractioned with various solvents in order of increasing polarity, starting with the least polar solvent, n-hexane
followed by chloroform, ethyl acetate and n-butanol.
As shown Table 4, Zingiber officinale Rosc (Khing) exhibited antioxidant activities with the range of 1.10 to 2.23 mg/mL in n-hexane
, chloroform, and butanol extracts.
Fucoxanthin content was analyzed using HPLC in ethanolic extract, n-hexane
, ethyl acetate and ethanolic fraction of Turbinaria decurrens.
Figure (5b, 5c) showed heterogeneous, porous surface morphology of Fe-doped TiO2 nanoparticles synthesized in acetonitrile and n-hexane
Among them, A2 exhibited high antioxidant activity and A3 also had relatively high antioxidant activity; therefore, second fractionation of A2 and purification of A3 by HPLC were performed by silica-gel semipreparative column (Wakosil 5SIL, Wako Chemicals Co.), eluted with n-hexane
and ethyl acetate (50% : 50%), and A2 gave four compounds based on peaks: AS1 (2.5 mg), AS2 (2.9 mg), AS3 (3.5 mg), and AS4 (5.2 mg), and A3 gave single compound (2 mg).
and ethyl acetate were used as solvents, respectively, the inverted micelle phases immediately separated (within 1 hour) into micelle-poor and micelle-rich phases that formed densely packed aggregates, and most micelles were destroyed (Figure 2) as n-hexane
has lower polarity (polarity index = 0.1) and ethyl acetate higher solubility in water (8.7 g/100mL) than chloroform (polarity index = 4.1 and solubility in water = 0.81 g/100mL).
The isolating process of FFAs was conducted in separatory funnel using n-hexane
as solvent to extract FFAs and residual glycerides.
A mass of 160 g of powdered plant material was subjected to a serial exhaustive extraction using the maceration method in 3000 ml of n-hexane
, dichloromethane, methanol, and water (starting with less polar to more polar solvent).
atrophaeus was susceptible to all the extracts except n-hexane
. Against B.