Nephelometric Analysis

Nephelometric Analysis

 

a chemical method of quantitative analysis based on the measurement of the intensity of the light scattered by disperse systems. It was originally used in analyzing certain naturally turbid objects, for example, river water. Later, artificial suspensions were used to determine solute concentration. For example, sulfate concentration in water is determined by first using BaCl2 to obtain a BaSO4 suspension; the intensity of light scattering in this medium is next measured with a nephelometer; and, finally, the SO42- ion concentration is found by means of a calibration curve. Nephelometric analysis is currently applied to determine the concentration of petroleum products in water, as well as in the examination of various other products, such as pharmaceuticals and foods. It is a suitable method for determining concentrations of 10-5 to 10-4 percent with an accuracy of approximately ± 5 percent.

Nephelometric analysis is done with special devices called nephelometers, which measure the scattered light through an ocular positioned at a 90° angle to the incident light beam. In addition, certain photoelectric colorimeters, for example, FEK-N-57 and FEK-56–2, are currently being adapted for use as nephelometers.

REFERENCES

Yoe, J. H. “Fotometricheskii khimicheskii analiz,” vol. 2: Nefelometriia, Moscow, 1936.
Fiziko-khimicheskie metody analiza: Prakticheskoe rukovodstvo, 2nd ed.
Edited by V. B. Aleskovskii and K. B. Iatsimirskii. Leningrad, 1971.
Váńa, J. Analizatory gazov i zhidkostei. Moscow, 1970.
Lur’e, Iu. Iu., and A. I. Rybnikova. Khimicheskii analiz proizvodstvennykh stochnykh vod, 2nd ed. Moscow, 1963.

IU. A. KLIACHKO

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The subject of procurement is the purchase of biochemical analyzer to the lab OKBH Rheumatology Institute and continuous supply of reagents for the implementation of relevant biochemical, potentiometric and turbidimetric / nephelometric analysis, and associated process chemicals and consumables.
Quantification of IgE and cytokines in serum: Serum concentrations of IgE, cytokines such as IL-4, IL-10 and complement proteins such as C3c and C4 were quantified by nephelometric analysis using Nephelometer (BN ProSpec, Dade Behring, Marburg, Germany).
Though IL-4 was not detectable in all CE patients and controls, nephelometric analysis to study the Th2 cell activation among the patients with CE seems to be more authenticated than previous studies in vitro (11) as well as in vivo (30,31).
We therefore investigated whether lithium-heparin plasma can be used for nephelometric analysis of a whole array of serum proteins.
The subject of procurement is the purchase of biochemical analyzer to the lab OKBH Institute of Rheumatology in Prague and continuous supply of reagents for the implementation of relevant biochemical, potentiometric and turbidimetric / nephelometric analysis, operational and related chemicals and consumables.
The value, however, is likely to be artificially increased because of the large size of the aggregate, which may influence interactions with the polyclonal antisera differently from the monomeric calibrator in the nephelometric analysis.
Acquisition of biochemical analyzer in the laboratory Department of Clinical Biochemistry and Haematology Institute of Rheumatology in Prague and continuous supply of reagents for the implementation of relevant biochemical and turbidimetric / nephelometric analysis and related process chemicals and consumables.
Light chain myeloma: correlation of serum nephelometric analysis for the quantitation of immunoglobulin free light chain with urine Bence Jones protein [Abstract].
Polymer enhancement of automated immunological nephelometric analysis, as illustrated by determination of urinary albumin.
Values obtained by nephelometric analysis of the samples from the male patients were compared with reference intervals (see above) and found to be statistically significant (Wilcoxon scores) in the decreased concentrations of transferrin (1.