Nessler's reagent


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Nessler's reagent

[′nes·lərz rē‚ā·jənt]
(analytical chemistry)
Mercuric iodide-potassium iodide solution, used to analyze for small amounts of ammonia.
References in periodicals archive ?
The mixture solvent contains ethyl acetate that acts as a solvent and a specific reagent for paracetamol (ferric chloride, Folin-Ciocalteu reagent, Nessler's reagent, and Lieberman reagent).
The colorimetric sensing ability of reagent of paracetamol (ferric chloride, Folin-Ciocalteu reagent, Nessler's reagent, and Lieberman reagent) was investigated by adding paracetamol to the reagent solution.
Table 1 shows that the solution color changed after paracetamol was added to the Fe[Cl.sub.3], Folin-Ciocalteu, and Nessler's reagent. Fe[Cl.sub.3] gives blue and black colors, and the Folin-Ciocalteu gives a dark blue color when this reagent is reacted with phenol on paracetamol.
All of the specific reagents for paracetamol (ferric chloride, Folin-Ciocalteu reagent, and Nessler's reagent) are water soluble.
In addition, the isolate produced ammonia as indicated from the color change to yellow of the culture after adding Nessler's reagent. The isolate also solubilized phosphate as indicated from the zone formed on PKV medium and the pH change of the culture in Pikovskaya's broth.
Standard and sample tubes were prepared and treated with Nessler's reagent. Absorbance values which obtained from each groups were calculated with following formula.
The reaction between the ammonia in the reaction mixture and Nessler's reagent was indicated by the formation of an orange colored solution.