Keywords: Nylon 6,6; Graphene nanoplates; Neutral red
chloride dye; Adsorption.
MDCK-C11 cells viability was determined by the neutral red
method, which evaluates the cell endocytic ability by measuring the absorbance of the dye.
working solution (30 [micro]L) was added to the area containing the attached cells and a cover slip was applied.
Phenazine dyes; Neutral Red
, Safranin T, and Janus Green were obtained from Merck (Darmstadt, Germany).
According to the previously mentioned experimental grouping, after 24 h of cocultivation, the culture medium was replaced with PBS containing 0.05% neutral red
(Sigma) for another 30-40 min.
The cytotoxic potentials of Co[Fe.sub.2][O.sub.4]-NPs were determined by MTT and neutral red
uptake (NRU) assay based on different cellular mechanisms.
For the MTT and Neutral Red
assays, cells were plated at the density of 5000 per well in a 96-well microplate (7-8 wells were determined for each experimental group).
The stock solution of neutral red
(NR) dye was prepared by solubilizing 0.002 g in 1 mL of DMSO.
Thus, the phagocytic activity of polysaccharide-activated macrophages was studied by the uptake of neutral red
. As shown in Figure 2(c), RGP1, RGP2, RGP3, and RGP4 could significantly stimulate the phagocytic activity of RAW 264.7 cells compared with control group ([greater than or equal to] 10 [micro]g/mL, [greater than or equal to] 100 [micro]g/mL, 1000 [micro]g/mL, and 1000 [micro]g/mL, resp., p < 0.05).
The limited cuticle sclerotization of termites permits the use of histological markers such as Sudan red-7B, Nile blue-A or Neutral red
(Su et al., 1991; Evan, 1997).
It was also interesting to see that an official statement by the neutral Red
Cross could not verify or deny the systematic starvation of Madaya's people.
On the other hand, word from neutral Red
Cross officials that concentration camp inmates would be aboard the vessels was, in fact, passed on to 2nd TAF on May 2, but the squadrons involved were not advised.