nigrosine

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Related to Nigrosin: nigrosin stain

nigrosine

[′nī·grə‚sēn]
(materials)
Any of a group of blue or black azine dyes used for coloring inks, shoe polish, leather, and wood; can be water-, alcohol-, or oil-soluble.
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References in periodicals archive ?
The eosin nigrosin stained slide was used for the estimation of morphological defects under 100x oil emersion lens of light microscope.
Morphological observation was determined from a total count of 100 spermatozoa in smears obtained with nigrosin and eosin for live-dead count.
Microscopic examination included 10% KOH mount, Gram stain, wet mount & Negative staining by Nigrosin depending upon the type of sample.
Evaluation of the one step eosin nigrosin staining technique for human sperm vitality assessment.
Following a 30 second incubation, 150 [micro]L of ten percent nigrosin solution is added, and mixed.
Viability testing requires a very simple two-step staining procedure, using eosin-Y as the stain and nigrosin as a counterstain.
The strips were stained with nigrosin (Acid Black 2), dried, and made transparent with microscope immersion oil that had a refractive index of 1.474.
Determination of dead spermatozoa: For dead sperm ratio, Eosin and Nigrosin stain was prepared, mixing 1% Eosin and 5% Nigrosin in 3% sodium citrate dehydrate solution (Zemjanis, 1970).
KG, Karlsruhe, Germany) to four parts 10% nigrosin aqueous solution [Sigma-Aldrich, USA]) using a glass rod to produce a smear on the slide.
Catalase negative colonies that showed the presence of capsule on nigrosin stain and were optochin sensitive was further confirmed by bile solubility test.