The aim of the project was to focus on the detection of healthy carriers of piscine Nodavirus due to the importance of this virus in Mediterranean and the North African aquaculture .
For this purpose, 13 species of wild fish were investigated for the presence of the Nodavirus RNA2 segment during a routine survey conducted between January-July 2014.
Nodavirus screening was performed by qRT-PCR using the Applied Bio system 7500 machine and reaction conditions were realized according the validated protocol of Panzarin et al.
Partial RNA2 nucleotide sequences of 32 Nodavirus isolates available in GenBank were included in the analyses.
Apparent prevalence of Nodavirus within the different fish families showed that the Sparidae and the Carangidae families present a great susceptibility to the virus (with a detection rate of approximately 92% and 60%, respectively).
To date, nodavirus has been isolated from approximately 50 fish species worldwide and several studies have established that this virus is globally distributed in the environment [15,19,20].
Isolation in cell culture of nodavirus from farmed Atlantic halibut
Pathological changes in juvenile Atlantic halibut Hippoglossus hippoglossus persistently infected with nodavirus.
Cell culture isolation of piscine neuropathy nodavirus
from juvenile sea bass, Dicentrarchus labrax.
Rapid detection of Macrobrachium rosenbergii nodavirus
(MrNV) and extra small virus (XSV), the pathogenic agents of white tail disease of Macrobrachium rosenbergii (De Man), by loop- mediated isothermal amplification.
e) Otras enfermedades detectadas solo a nivel regional y con baja prevalencia (2,5 a 7,5%), fueron mionecrosis infecciosa (IMN) en Brasil, sindrome de Taura (TS) en Venezuela, estreptococosis en Guatemala, Litopenaeus vannamei nodavirus (LvNV) en Belice y Baculovirus penaei (BP) en Nicaragua, concordando en lo general con la literatura reportada para estas enfermedades en Latinoamerica [5, 9, 11, 18, 26, 31, 32, 35, 36, 52].
vannamei nodavirus (LvNV) detectado en Belice se encontraron cuerpos de inclusion citoplasmaticos basofilicos en las celulas del musculo y areas con necrosis coagulativa de las fibras musculares estriadas.