A liquate concentration of (S1) 53.65 ppm of AgNPs was Non-Competitive inhibition
for GOT enzyme activity.
In case of non-competitive inhibition
Vmax is decreased while Km is not effected, while in un-competitive type of inhibition both Vmax and Km are decreased.
(Relevant links: 'enzyme function and inhibition https://www.youtube.com/watch?v=PILzvT3spCQ: 'Competitive and non-competitive inhibition
. mp4' https://www.youtube.com/watch?v=99X1u3pTQ0s)
However, a similar [K.sub.m] values found for both experiments indicates that zinc actions on iron uptake are a non-competitive inhibition
. If it is non-competitive inhibition
, increasing the concentration of iron still does not allow the maximum [V.sub.max] value to be reached.
The models tested included pure and partial competitive inhibition, non-competitive inhibition
, mixed-type inhibition, and uncompetitive inhibition [10,11].
The [IC.sub.50] values of the AFA extract (concentration 10 mg/ml), AFA-PC and AFA-MAAs were 6.4 [micro]l/ml, 1.33 [micro]M and 1.98 [micro]M, respectively, evidencing a mixed-type of inhibition for the AFA extract ([K.sub.i] 0.99 [micro]l/ml), a non-competitive inhibition
for AFA-PC ([K.sub.i] 1.06 [micro]M) and a competitive inhibition for AFA- MAAs ([K.sub.i] 0.585 [micro]M).