Northern blotting

(redirected from Northern blot)
Also found in: Dictionary, Medical, Wikipedia.

Northern blotting

[¦nȯrth·ern ′bläd·iŋ]
(genetics)
Method of ribonucleic acid (RNA) detection and identification in which the intact RNA is separated by size via gel electrophoresis, transferred (blotted) onto nitrocellulose or nylon paper, and then hybridized with labeled DNA probes.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
Southern blot and Northern blot hybridizations were performed as described previously by Lee et al.
Image bands from Western blot, Northern blot, and PCR/qPCRs were quantified using Image J software (NIH website).
In order to assess cell binding and internalization of the selected aptamers without the need for labeling, we performed Northern blot analysis.
One empty vector control and five Ubi-hpCP:Hc-Pro primary transgenic lines (T0) were analyzed for transgene copy number by Southern blot hybridization and for the accumulation of siRNAs by Northern blot hybridization.
Sensitive and specific detection of microRNAs by northern blots analysis using LNA- modified oligonucleotide probes.
For Northern blot analysis, total RNA was extracted from leaves using Trizol reagent (Invitrogen), and hybridizations were carried out with [[[alpha].sup.32] P] dCTP-labeled PHYB probe.
Nitrocellulose membranes for Western blot and nylon membranes for Northern blot were purchased from Invitrogen (Carlsbad, CA) and NEN Life Science Products (Boston, MA), respectively.
Transgene expression was first analysed in back skin, brain, and liver of all transgenic lines by northern blot using a [sup.32]P-labelled oligonucleotide A probe and a U6 RNA (a ubiquitously expressed component of the spliceosome) probe for loading normalization.
Small RNAs were isolated using the mirVana miRNA isolation kit (Ambion Austin, USA) and analyzed by Northern blot hybridization according SequaGel Sequencing system Kit (EC-833) (National Diagnostics Atlanta, USA) according to manufacturer's instructions.
Expression patterns of cPdcl2 during testicular and ovarian development in chickens were examined by quantitative real-time PCR (qRT-PCR), Northern blot hybridization, and in situ hybridization.
Full browser ?