Northern blotting

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Northern blotting

[¦nȯrth·ern ′bläd·iŋ]
(genetics)
Method of ribonucleic acid (RNA) detection and identification in which the intact RNA is separated by size via gel electrophoresis, transferred (blotted) onto nitrocellulose or nylon paper, and then hybridized with labeled DNA probes.
References in periodicals archive ?
Over expression of MsHsp23 transgenic alfalfa plants: We confirmed the expression of four lines (Tg1, Tg3, Tg4 and Tg7) by Northern blot analysis.
Sobre estos datos y con los resultados de expresion mediante Northern blot, se considera que el gen de guayaba pertenece al perfil de expresion 1, al igual que LeEXPAI.
Out of six miRNAs (161, 168a&b, 171a, 159, 167a&b, and 172a) selected for further validation, four miRNAs 161, 167a&b, 168a&b, and 171a showed a good correlation between the array and Northern blot signal and two miRNAs 159 and 172a display higher expression (Northern blot) in the mock samples then in the infected plants as seen on the array.
Because the relative expression levels of cPdcl2 were high in the testis of 24-weeks-old chickens, we further examined the specificity of cPdcl2 in several tissues of 24-week-old chickens using Northern blot.
Northern Blot Analysis of the HVA1 Gene in R3 Progeny Plants
Northern blot analysis of the expression of the putative FAMTase in mandibular organs from crabs at different stages of ovarian development showed that the relative expression of FAMTase, normalized using an 18S rRNA probe, was significantly higher in pre-vitellogenic crabs than in animals in the early stages of vitellogenesis (Fig.
Powerful and continually evolving methods, such as short-read sequencing (RNA-seq), microarray analysis, quantitative real-time RT-PCR, as well as traditional methods for differential gene expression studies using multiplex endpoint PCR and northern blot analysis are employed by scientists to analyze gene function, identify new therapeutic and diagnostic targets, and to map pathways involved in development and disease.
MEASUREMENTS AND MAIN RESULTS: Theaflavin inhibited tumor necrosis factor-alpha-mediated interleukin-8 gene expression, as measured by luciferase assay and Northern blot analysis, at concentrations of 10 and 30 microg/mL.
All PCR products (amplified from the coding region of their respective genes) were also used as probes for the southern and northern blot analyses described below.
Northern blot hybridization experiments were carried out to verify the microarray results, using the same total RNA samples prepared for microarray studies.
Northern blot analysis from the study showed that mRNA levels for these genes were upregulated when the C.
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