Northern blotting


Also found in: Medical.

Northern blotting

[¦nȯrth·ern ′bläd·iŋ]
(genetics)
Method of ribonucleic acid (RNA) detection and identification in which the intact RNA is separated by size via gel electrophoresis, transferred (blotted) onto nitrocellulose or nylon paper, and then hybridized with labeled DNA probes.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
Microarray Analysis Market Analyzed By Technology (DNA Microarray, PCR, NGS, SAGE, and Northern Blotting), Consumables (DNA Chips, DNA Microarrays, Protein Microarrays, Cellular Microarrays, Chemical Compound Microarrays, Antibody Microarrays), Services (Gene Profiling and Bioinformatics) and Applications (Research, Drug Discovery and Diagnostic) - Global Forecast till 2023
The tools classified as microarrays, next generation sequencing (NGS), quantitative real time PCR (qRT-PCR), northern blotting, in situ hybridization (ISH).
Based on techniques, the market is segmented into extraction tools, qRT-PCR, NGS, microarray, functional analysis tools (such as miRNA mimetics, inhibitors, and target site blockers), and others (such as labeling, ISH, and northern blotting tools).
Cells were then starved in DMEM serum-free overnight and stimulated with 25 [micro]M LPA (+) or vehicle (-) for 8 hrs, followed by Northern blotting for DR6 mRNA expression.
The earliest technique used for detection of RNA for the study of Gene expression was Northern Blotting. The technique involves the use of electrophoresis to separate RNA samples by size and to detect the target RNA with a hybridization probe complementary to the target RNA sequence.10The probes are composed of nucleic acids having a complementary sequence to all or part of RNA to be isolated.
Among the topics are detecting changes in global genome methylation using the cytosine-extension assay, isoschizomers and amplified fragment length polymorphism for detecting specific cytosine methylation changes, northern blotting techniques for small RNAs, cloning new small RNA sequences, cDNA libraries for virus-induced gene silencing, and detecting and quantifying DNA strand breaks using the random oligonucleotide primed synthesis (ROPS) assay.
The recombinant DNA was labeled with a digoxigenin RNA labeling kit (Roche) to prepare sense and antisense cRNA probes for Northern blotting and in situ hybridization.
The expression level of each gene in both the yeast and mold morphotypes of four Hc strains was examined by northern blotting and realtime PCR.
The PRSS11-L mRNA is widely expressed in several tissues throughout the body by multi-tissue Northern blotting. The full-length PRSS11-L cDNA, was cloned, expressed and purified.
In the latter study, differential display PCR was used, and it is not known if the affected transcripts were directly or indirectly regulated by 4-t-octylphenol or [E.sub.2]-As quantified in these latter studies using reverse Northern blotting, changes in several hypothalamic mRNAs induced by waterborne environmentally relevant levels of octylphenol in these studies were approximately 2-fold.
For Northern blotting, about 10 [micro]g of total RNA from individual tissues was electrophoresed on a formaldehyde/1% agarose gel for 3 h at 75 V.
Because Northern blotting detected no wild-type LDHA mRNA, the occurrence of some mutations in the LDHA gene was suspected.
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