LPL mRNA levels were determined by Northern blotting
and laser scanning of autoradiographs.
It is a more sensitive method to quantify gene expression than conventional methods used for mRNA evaluation, such as Northern blotting, dot-blot analysis, and the RNase protection assay.
The assay is a rapid (17) and sensitive method to quantify gene expression and requires smaller amounts of breast tissue than conventional methods, such as Northern blotting (18).
The purified RNA is of the highest integrity, and can be used in a number of downstream applications including Real-Time PCR, reverse transcription PCR, Northern blotting
, RNase protection and primer extension, and expression array assays.
We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting.
Verification of differential gene transcription using virtual northern blotting.
The protein and mRNA contents of UGT2B1 also were not detected in the fetal rat liver, but a developmental increase in newborn rat liver was detected by Western and Northern blotting
Neither genomic Southern blotting nor Northern blotting
of tumor RNA can be performed successfully with the degraded nucleic acids obtained from fixed and embedded tissue specimens.
Ready-to-use SuperSignal(R) Substrates, which are designed for use with horseradish peroxidase-labeled (HRP) reporter molecules immobilized on membranes or on microplates, emit an intense, long duration signal when they come into contact with antigen during Western, Southern or Northern blotting
or enzyme-linked immunosorbent assays.
We next examined the effects of cadmium on the transcript level of bcl-2 by Northern blotting
, comparing with that of c-myc as the immediate early gene.
Although duplicate performance of experiments may seem time consuming, our experience has shown that the extra effort expended at this stage is more than repaid by reducing unnecessary cloning, sequencing, and Northern blotting
later in the process.