Northern blotting


Also found in: Medical.

Northern blotting

[¦nȯrth·ern ′bläd·iŋ]
(genetics)
Method of ribonucleic acid (RNA) detection and identification in which the intact RNA is separated by size via gel electrophoresis, transferred (blotted) onto nitrocellulose or nylon paper, and then hybridized with labeled DNA probes.
References in periodicals archive ?
Based on techniques, the market is segmented into extraction tools, qRT-PCR, NGS, microarray, functional analysis tools (such as miRNA mimetics, inhibitors, and target site blockers), and others (such as labeling, ISH, and northern blotting tools).
The earliest technique used for detection of RNA for the study of Gene expression was Northern Blotting.
It is classified into PCR, sequencing, northern blotting, southern blotting, molecular cloning, and other nucleic acid applications.
Among the topics are detecting changes in global genome methylation using the cytosine-extension assay, isoschizomers and amplified fragment length polymorphism for detecting specific cytosine methylation changes, northern blotting techniques for small RNAs, cloning new small RNA sequences, cDNA libraries for virus-induced gene silencing, and detecting and quantifying DNA strand breaks using the random oligonucleotide primed synthesis (ROPS) assay.
The recombinant DNA was labeled with a digoxigenin RNA labeling kit (Roche) to prepare sense and antisense cRNA probes for Northern blotting and in situ hybridization.
The expression level of each gene in both the yeast and mold morphotypes of four Hc strains was examined by northern blotting and realtime PCR.
The PRSS11-L mRNA is widely expressed in several tissues throughout the body by multi-tissue Northern blotting.
2]-As quantified in these latter studies using reverse Northern blotting, changes in several hypothalamic mRNAs induced by waterborne environmentally relevant levels of octylphenol in these studies were approximately 2-fold.
For Northern blotting, about 10 [micro]g of total RNA from individual tissues was electrophoresed on a formaldehyde/1% agarose gel for 3 h at 75 V.
Because Northern blotting detected no wild-type LDHA mRNA, the occurrence of some mutations in the LDHA gene was suspected.
Applications include gene expression, single nucleotide polymorphisms analysis, genotyping, drug screening, in situ tissue analysis, immunohistochemical analysis, cellular receptor binding detection, Western and Northern blotting, flow cytometry, microfluidics, microarrays, and multiple clinical diagnostic applications.
When checked by a number of methods including quantitative RT-PCR (6, 35), Northern blotting (33, 34, 36), and protein expression (33, 34), most differentially expressed genes have been confirmed.
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