The ulcerated area showed abundant epithelioid cells in loosely held stroma showing highly dysplastic features of pleomorphism, altered nucleocytoplasmic ratio
, and atypical mitoses (Figure 7(c)).
A biopsy performed from the margin of the ulcer showed keratin pearls, nuclear hyperchromatism and pleomorphism with increased nucleocytoplasmic ratio
of the squamous epithelial cells confirming it as well-differentiated keratinizing SCC (Figure 3).
In adenomas showing moderate dysplasia there is focal loss of nuclear polarity, an increase in the nucleocytoplasmic ratio
and further loss of mucin production.
The criteria for malignancy included: I) disrupted nucleocytoplasmic ratio
; II) prominent nucleoli, increased in size, with irregular configuration; III) an increased number of nucleoli; IV) irregular nuclear membrane; V) nuclear hyperchromasia; VI) coarse chromatin; VII) decreased cell adherence; VIII) necrosis; and IX) mitotic figures.
Peripheral blood neutrophils  are showed striking dysplastic  features, which included detached nuclear fragments, acquired Pelger-Huet anomaly chromatin clumping, neutrophils with strangely shaped nuclei, and a high nucleocytoplasmic ratio
The epithelium within this pseudopapillary lesion showed an increased nucleocytoplasmic ratio, round large nuclei, and prominent nucleoli (Figure 1, B).
In summary, this case illustrates that several of the key criteria used for a diagnosis of TIC can be seen in papillary syncytial metaplasia of tubal mucosal endometriosis, including marked epithelial stratification with loss of polarity, increased nucleocytoplasmic ratio, and rounded nuclei with chromatin clearing and prominent nucleoli.
Imprint smear from the ulcer showed mainly necrosis and few cells with high nucleocytoplasmic ratio
. Further details were obscured by necrotic debris.
The cells were cuboidal in the hyperplastic and papillary areas, with an increased nucleocytoplasmic ratio
, round bland nuclei with low mitotic activity, and inconspicuous nucleoli (Figure 1).
Important cytological criteria useful in distinguishing HCC from non-neoplastic liver are increased nucleocytoplasmic ratio
, arrangement of tumor cells in trabecular pattern and atypical naked hepatocytic nuclei.
Although occasional cells showed ultrastructural intermediate features between the undifferentiated and glandular cell types (large size and high nucleocytoplasmic ratio
but a well-developed endoplasmic reticulum, a prominent Golgi complex, and few secretory granules), in general, there was an abrupt transition between the 2 components.
Individual cells were uniform sized with eosinophilic cytoplasm, high nucleocytoplasmic ratio
and increased mitotic activity (Figure 2).