Glycosylation sites analysis showed that one N-linked glycosylation sites (N20) and eight O-linked glycosylation
sites (T22, T24, T25, S33, S38, T41, T46 and S269) were found in the amino acid sequence according to NetNGlyc 1.0 Server and NetOGlyc 4.0 Server, respectively.
occurring with the addition of aO-mannose is the only form of O-linked glycosylation
in yeast but also occurs in the brains of higher eukaryotes [68, 69].
Bertozzi, "The chemistry and biology of mucin-type O-linked glycosylation
" Bioorganic and Medicinal Chemistry, vol.
However, large comparative studies have failed to reveal major differences between BNP and proBNP measurements in terms of overall clinical performance, although plasma measurement based on assays directed against the N-terminal proBNP fragment is greatly influenced by the degree of O-linked glycosylation
. Clearly, this issue is far from settled, and our present perception of "normal" concentrations of the biosynthetic products may have to be reconsidered.
The dystroglycanopathies: the new disorders of O-linked glycosylation
. Semin Pediatr Neurol 2005; 12: 152-8.
(11) reported O-linked glycosylation
of proBNP that hampers antibody recognition (12).
Of particular interest to later discussion are signals shifted up in mass by 162, 203, and 657 Da, suggesting glycation (Glyc), O-linked glycosylation
(GalNAc), and a second O-linked glycoform ([NeuNAc.sub.1][Gal.sub.1][GalNAc.sub.1] trisaccharide) (TS), respectively.
Thus, it is possible that the modification preventing antibody binding is O-linked glycosylation
. Although direct evidence was not provided, Seferian et al.
The effects of O-linked glycosylation
on the bioactivity of many signaling molecules, particularly hormones and cytokines, and a relatively small number of enzymes, have been described.