ORF

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ORF

[ȯrf or ¦ō¦är′ef]
(cell and molecular biology)
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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In previous study [18], a classified group of 39,579 ORFs was obtained from comparison of 164,644 ORFs from the metagenomic data of bacteria in goats' rumen against the NCBI non-redundant protein (NR) database using the BLASTX algorithm with an E-value of less than [10.sup.-5].
Therefore, it is important to develop the computational methods for identifying the coding ORFs from the metagenomics short reads.
ORFs for the synthesis of UDP-2,3-bis(3-hydroxymyristoyl)glucosamine biosynthesis, such as acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase, RWLH04242, UDP-3-O-acyl-N-acetylglucosamine deacetylase, (RWLH04143) and UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase (RWLH04240) were identified.
Major General James Wright fellows at the College of William and Mary's Mason School of Business undertook a capstone project for determining the validity of the Army's operational readiness float (ORF) program as a readiness enabler.
Table 1: Results of the qPCR assay targeting ORF 0047 and ORF 0072 unique to pBC210 using bacterial and soil DNA extracts as template.
2 can be regarded as revealing the asymptotic low-ability behavior of the putative ORFs. The conditions illustrated in Fig.
Phylogenies constructed from individual ORFs including ORF 1ab, S, E, M, and N demonstrated a largely consistent phylogenetic position of the novel human betaCoVs, except for a small discordance in the order of branching between novel human betaCoV, HKU4, and HKU5 lineages (Figure 3, panels B-F, Appendix).
PCR amplification of ORF 62 was performed on all the clinical specimens and the vaccine strain using the PKVL6U-PKVL1L (genome position 106036-106284) set of previously-published primers [12].
Based on analysis of some 6,000 yeast genes, the report noted that ''yeast (eukaryotic) ORFs related to the nucleus share their origins with archaeal ORFs, whereas ORFs that are related to the cytoplasm share their origins with bacterial ORFs.''
Using GATU software (10), we identified 166 ORFs (online Technical Appendix, www.cdc.gov/EID/content/17/4/599-Techapp.htm).
To further characterize the tetM genes, the open reading frame (ORF) was completely sequenced by using 16 selected strains: 9 isolates from humans and 1 from a pig from the Sichuan outbreak; 3 from sporadic infections in Guangxi, Jiangsu, and Guangdong; 1 from a diseased pig in Jiangxi Province in 2005; and 2 from the Jiangsu outbreak in 1998 (Table 1).