After calibration variables like cellular diame- ter(CD) and nuclear diameter (ND) of the 25 cells in each smear were measured by using calibrated ocular micrometer
fixed in eye piece of microscope on 40 x (Fig 1).
In view of the exponential technological progress and, besides the tedious and monotonous use of the ocular micrometer
, make the use of the instrument in question disappearing especially with the advent and, the generalization of high performance microscopes.
Measurements were done using an ocular micrometer
in a stereoscopic microscope at a magnification of 15X.
Part 2 Basic Micrometry and Calibrating an Ocular Micrometer
For an index to age we measured the length of a 1st upper incisor (I1) along its lateral edge from the cingulum to the tip in ocular micrometer
lines (= 0.033 mm each) on dermestid-cleaned skulls.
Without the use of an ocular micrometer
, oocysts of Cyclospora might be easily confused with those of Cryptosporidium or other fecal artifacts that stain acid-fast positive, as was the case in a pseudo-outbreak of cyclosporiasis reported in Florida (6).
Oocysts were measured using a calibrated ocular micrometer
and are reported in micrometers as means, followed by the ranges in parentheses.
Measurements were taken using an ocular micrometer
and line illustration were made using a drawing tube attached to the compound microscope.
was calibrated with stage micrometer and used for the measurement of epithelial height with 40 X objective lens.
Beetle abdomens were macerated, and free sporophorous vesicles were examined under phase contrast microscopy at 400x magnification and measured with a calibrated ocular micrometer
(n = 125).
The pollen was examined with a microscope (Olympus model BH-2), connected to a TV screen, measured with ocular micrometer
at 1000X (using immersion oil).