To identify the differentiated oligoprogenitor cells and oligodendrocytes
, immunocytochemistry technique was done.
In the CNS, cells called oligodendrocytes
create and lay down the myelin sheath that surrounds neurons.
To address this problem, the team developed a rapid and highly efficient method for generating OPCs and oligodendrocytes
from pluripotent stem cells from any genetic background, providing new access to these relatively inaccessible brain cells in healthy and diseased states.
For all the studies confirming the positive effects of 9-cis-RA and 1,25[(OH).sub.2][D.sub.3] on the differentiation of oligodendrocytes
, the inhibitory effects of these components on signaling pathways have yet to be thoroughly explored.
Convelo Therapeutics is developing brain-penetrant small molecule therapeutics that stimulate oligodendrocyte
differentiation and myelin regeneration from oligodendrocyte
precursor cells (OPCs).
MAG, another MAI [52,72, 73], is a minor component of mature, compact myelin, enriched in the periaxonal membrane of the myelin sheath and is expressed by oligodendrocytes
and Schwann cells.
Fossati has developed a unique way to take human skin cells, create iPSC, convert those into oligodendrocytes
and, she hopes, use the oligodendrocytes
as a better model for studying myelination in MS and evaluating potential MS stem cell therapies.
We used coronal sections containing the damaged striatum located at 1.2 mm posterior to bregma to count the number of activated macrophages/ microglia (CD68+ cells), MHC-II+ cells, pathological oligodendrocytes
(Tau-1+ cells), Nogo-A+ cells, and astrocytes (GFAP+ cells) per field.
The third glial class, oligodendrocytes
, supports neurons by wrapping the neurons' long, wiry fibers called axons in myelin, a fatty protective substance better known as the brain's white matter.
were double-labeled with AnnexinV-FITC/propidium iodide (PI) by an Apoptosis Detection Kit (KeyGEN BioTECH, Beijing, China).
The second study identified a process that centers on oligodendrocytes
, cells that produce the protective myelin sheathing that coats the brain's wiring.