The most commonly practiced modified Ziehl Neelsen (ZN) acid fast staining is a key player in the detection of Cryptosporidium oocysts
in fecal smears (Rekha et al., 2016).
People can help reduce the spread of Toxoplasma by keeping their cats inside and disposing of cat faeces in a bag in the trash, not outdoors or in the toilet because wastewater treatment is not effective in killing oocysts
Normally, most birds pass small numbers of oocysts
in their droppings without apparent ill effects.
Given its impact, it is little wonder that Cryptosporidium is treated as a bioterrorism agent, and the European Water Directive insists on shutting down the distribution plants should the oocysts
be found in drinking water.
Inactivation of Cryptosporidium parvum Oocysts
and Clostridium perfringens Spores by a Mixed-Oxidant Disinfectant and by Free Chlorine.
can be highly resistant to chemical disinfectants, including chlorine.
For examination of Cryptosporidium oocysts
, the faecal smears were prepared and stained by Modified Ziehl-Neelsen technique using ZN Acid Fast Stains Kit (Himedia, India) (Henriksen and Pohlenz, 1981).
Infected animals can shed a huge number (108-109/g) of oocysts
(Ortegamora and Wright, 1994), which cause infection in humans and animals by contaminating the surface water of routine usage (Maurya et al., 2013).
The Cornell Feline Health Center says that cats who eat flies and other bugs may get infected that way, too, if the insects have oocysts
on their bodies.
If the unsporulated oocysts
come into contact with moisture, they sporulate (infectious) and once ingested by other birds as they scratch and feed, they get infected.The oocysts
can also be transmitted through shoes, farm equipment, contaminated water and feeds.
(2012) argue, environments frequented by cats can be widely contaminated with oocysts
of T gondii and farms with a higher number of cats are generally the most contaminated.
We observed 15 (3.2%) samples positive for coccidian oocysts
out of 470 samples through microscopy.