The morphology of the composites was studied by optical microscopy
and transmission electron microscopy.
Analysis of isolated sperm samples at different dilutions showed the following results: Dilution 1:2 showed a poor agreement between flow cytometry and optical microscopy
(r = 0.33); dilution 1:4 showed a higher correlation coefficient (r = 0.81); the correlation was better (r = 0.96) with 1:8 diluted samples, and a sperm window was fixed (Fig.
To complement SEM analysis about homogeneity of the film, it was carried out the Optical Microscopy
. In this test the proteins are stained red and the fat globules were presented in white tones.
"As much of current cutting-edge bioresearch is moving away from before-and-after imaging of fixed cells and toward imaging dynamic cellular events as they happen, this is a significant advantage for optical microscopy
," says Joubert.
Surface Science Instrumentation 2007-08 Market Share Growth Rate Optical Microscopy
51.6% 5.5% Electron Microscopy 31.2% 7.7% Surface Analyzers 7.1% 3.3% Scanning Probe Microscopy 6.9% 10.6% Confocal Microscopy 3.1% 6.0% Total 100.0% 6.4% Surface Science Instrumentation Market Leaders Optical Microscopy
Olympus, Nikon Electron Microscopy Hitachi, JEOL Surface Analyzers Ulvac-PHI, Cameca (AMETEK) Scanning Probe Microscopy Veeco, SII Nanotech Confocal Microscopy Carl Zeiss, Leica (Danaher) 2006-09 Total Surface Science Instrumentation Market 2006 $4,019 2007 $4,263 2008 $4,536 2009 $4,830 Note: Table made from bar graph.
Drawn from many countries, specialists in the imaging dimension and in the biological dimension examine such topics as the basic concepts, high-speed AFM for observing dynamic processes in liquid, integrating AFM with optical microscopy
techniques, single-molecule AFM of cellular sensors, and nanosurgical manipulations of living cells.
Loiterman, Vice President and General Manager of Bruker's Stylus and Optical Metrology business, added: “ContourGT systems equipped with the award-winning AcuityXR technology have shown resolution of features below 130 nanometers in width, which is unprecedented in three-dimensional optical microscopy
. With AcuityXR, dimensional repeatability on narrow structures has been shown to improve by more than a factor of five, showing a true metrology benefit in semiconductor, medical and precision machining applications where feature sizes are rapidly shrinking, even as the demands for quality are increasing.”
JEOL USA, Inc., Peabody, Mass., in conjunction with the National Institute of Advanced Industrial Science and Technology (AIST), Japan, has developed a new microscopy tool--the ClairScope--that couples optical microscopy
with an atmospheric scanning electron micro scope (ASEM) in a novel open culture system that retains the samples' native state while under study.
Some optical microscopy
methods are available, but they require the use of special substrates, too.
Scientists in a wide range of biomedical specialties explain how the technologies of specific chemical and genetic labeling can be used in conjunction with optical microscopy
to analyze dynamically the spatial and temporal organization of proteins, lipids, and even messenger RNA in single living cells.
FEI Company and Max Planck Institute (MPI) of Biochemistry are collaborating on a correlative microscopy solution that enables scientists to acquire quickly and easily high-resolution transmission electron microscope (TEM) images of molecular entities found using optical microscopy
Nikon Instruments, Inc., Melville, NY, is a leader in the development of advanced optical microscopy
and digital imaging technology.