For the differentiation of BMSCs into osteoblasts
, BMSCs were cultured at a density of 1 x [10.
Derived from stem cells, osteoblasts
are responsible for the laying down of new bone matrix.
In the present study increase in osteoclast activity was associated with elevation of crosslaps and osteonectin concentration, bone turnover and osteoblasts
activity (osteocalcin concentration was also increased in weeks 4 and 8 after operation).
Enhancement of bone morphogenetic protein-2 expression and bone formation by coumarin derivatives via p38 and ERK-dependent pathway in osteoblasts
However, some reports have indicated that BMP2, a critical autocrine and paracrine growth factor that directs osteoblast
differentiation and bone formation, plays an important role in heterotopic ossification.
used carbon nano fibers as grown with pyrolytic outer layer (AG), and pyrolytically stripped carbon nano fiber (PS) for investigating the variation in the osteoblasts
behavior based on the surface chemical composition.
not only play a central role in bone formation by synthesizing several bone matrix proteins, but regulate osteoclast maturation through expression of soluble factors RANKL and M-CSF, resulting in bone resorption.
Histomorphometric parameters of medullary bone diameter, trabecular bone area, osteoid area, mineralized bone area, and osteoblast
area were measured.
Interstitial growth at a specialised area of long bone near the epiphysis known as the epiphyseal (growth) plate, where cartilage cells (chondrocytes) eventually enlarge and degenerate, and are replaced by osteoblasts
and osteocytes invading from the diaphysis towards the space created at the plate by the degenerating chondrocytes.
The prevailing model of osteology is that bones constantly undergo a remodeling process, and that the differentiation and functions of osteoblasts
are partially regulated by leptin through different central hypothalamic pathways.
Extraction of total RNA of osteoblasts
and reverse transcriptase PCR (RT-PCR)
The cytologic features of osteoblasts