Samples Catalase Test Oxidase Test
Gram staining LAB001-B Negative Negative Gram positive LAB002-C Negative Negative Gram positive LAB003-D Negative Negative Gram positive LAB004-E Negative Negative Gram positive LAB005-F Negative Negative Gram positive Table 2: Phenotypic identification of isolates using API 50 CHL Kits and API web.
The oxidase test
is used to differentiate among Gram-negative rods.
A spoonful of sugar -improving the sensitivity of the glucose oxidase test
strip method for detecting subclinical pulmonary aspiration of enteral feed.
cholerae resistance to O/129 makes bacteriologic identification more difficult (3,4), although this test is hardly used in the majority of Brazilian laboratories, with preference to oxidase test
when the objective is to analyze the behaviour of screening media and the antigenic characterization of O1 and O139 serogroups.
In contrast to other approaches, the MALDI BioTyper does not require any initial assessment like gram staining, oxidase test
of unknown samples, choice of PCR primers or usage of selective growth media.
All strains were tested positive for catalase test while negative for oxidase test
Then oxidase test
were done, for suspicious colonies utilaztion of sugars (glucose, lactose, maltose and sucrose) were used.
If Modified Oxidase test
was positive, it was identified as Micrococci.
Biochemical test Klebsiella Escherichia pneumonia coli Catalase test + ve + ve Oxidase test
- ve - ve simmon citrate test + ve - ve Urease test + ve Yellow/Yellow with gas production(-)ve TSI + ve + ve Indol test - ve + ve Motility test - ve + ve Methyl red test - ve + ve Vogas-proskaure test + ve - ve Gram stain - ve - ve Cell morphology On Macconkey agar Smooth, glassy and bacterial colonies translucent, rosy have a viscous/ pink on MacConkey mucoid appearance with the appearance of metallic sheen when growing on Eosin methylene blue.
Provisional identification of isolates was based on Gram stain and results of oxidase test
and API 20NE identification strip (Biomerieux, Marcy l'Etoile, France).