PCR


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PCR

(cell and molecular biology)
References in periodicals archive ?
Wise Guy Reports' (WGR's) recent report on the Digital PCR Market has revealed great that the said market can traverse several limitations and acquire extraordinary growth rate during the forecast period 2019 to 2025.
We will float tenders for the procurement of PCR kits asking the successful bidders to provide us with free machines, too,' manager of the KP Hepatitis Control Programme Dr Kalimullah Khan told Dawn.
5 [micro]L of DNA template was added to 25 [micro]L reaction mixture with 5 [micro]L of 5x PCR buffer, 200 [micro]M of deoxynucleoside triphosphate mix, 1 [micro]M of each primer, 3 mM of magnesium chloride, and 1U of Taq DNA polymerase (Promega Corporation, USA).
In each independent PCR assay, the test result was compared with the results for one positive and one negative control.
The company recently produced a mostly brown PP package with 60% PCR. The beauty industry typically shies away from packages of that color, but Combs said the product is a sales success and the package has won several awards.
The 1997 (Idaho Technology) and 1998 (Roche) commercial LightCycler systems introduced melting analysis, SYBR Green I, adjacent hybridization probes, rapid temperature cycling, and immediate data presentation to real-time PCR. Previously, the only other commercial instrument collected data once each cycle with batch analysis and display after PCR was complete.
The introduction of real-time PCR, which combined the use of fluorescently tagged probes with the utilization of PCR thermocyclers with fluorescent detectors, was another significant technological improvement.
The factors driving the growth of this market are increasing demand for innovative technology supporting early and accurate diagnosis of life threatening disease, rising demand for PCR in genetic and molecular testing, expiry of key players PCR patents, rising investments in the field of companion diagnostics, advent of digital PCR, increasing RT-PCR based food safety testing, increasing affinity for automated high throughput and robotic systems and multiplexed analytical strategies for improving sensitivity and speed of PCR technology.
Results: While at least one bacterial isolation was determined in 62 (31.5%) of all patients with culture method this number increased to 125 (63.5%) with multiplex PCR. The bacteria most commonly identified by PCR were S.
The salaries of PCR personnel will be withheld if they go on leave without intimating anyone for 10 days, and absentees will be placed under suspension if they fail to inform anyone for 30 days, the order further stated.
The specific sequence was amplified using an MTB DNA (PCR-fluorescence) diagnostic kit with Roche LightCycler PCR amplification (Roche, Germany).
SYBR Green I real-time PCR was carried out using SYBR Premix Ex Taq (TaKaRa) and the LightCycler 480 Real-Time PCR System (Roche Applied Science).