PPLO


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PPLO

References in periodicals archive ?
For sterility testing, no growth was observed on blood agar, thioglycolate and PPLO broth even after 14 days of incubation.
After 10 days of incubation of PPLO broth, RCM and blood agr plates inoculated with the vaccine at 37oC no aerobic or anaerobic bacterial growth was observed.
In order to obtain pure culture, a single colony was picked and sub-cultured to broth which was passed through a cellulose acetate membrane filter of 0.45u (Orange Scientific, Braine-I' Alleud, Belgium).Dilutions of the filtrate were then plated onto PPLO agar (Acumedia, Michigan, USA) to obtain isolated colonies.
urealyticum, NCTC 10177, National Collection of Type Cultures, London, UK) and a negative control (PPLO broth) were included in each PCR batch.
The swabs were cultured in pleuropneumonia-like organisms (PPLO) broth media at 37[degrees]C for 24 h., and then inoculated in PPLO media agar plate at 37[degrees]C for 1 week to examine the presence of Mycoplasma colonies (Evans et al., 2009).
A total of 300 samples, (150 sheep and 150 goats) were randomly collected from nasal discharge, pleural fluid and lung tissues, and streaked into Pleuropneumonia like organism (PPLO) medium for isolation and identification of Mycoplasma species.
pneumoniae (PPLO broth, HiMedia, Mumbai, India), and then transferred at 4[degrees]C to the Mycoplasma Laboratory of the Division of Microbiology, Tehran University of Medical Sciences, for immediate processing.