It is expected that the peptone
feeding approach utilized in this study would improve TGE yields in CHO cells and this method will be an attractive alternative to TGE in HEK-293 cells for the generation of recombinant proteins in preclinical research.
It is worth to mention that buffered peptone
water and tetrathionate broth were used for enrichment purpose.
MAC CONKEY AGAR: peptone
(20.0 g), synthetic detergent (5.0 g), sodium chloride (5.0 g), lactose (10.0 g), neutral red (0.07 g), agar (12.0 g).
Ten grams of sample and 100 mL peptone
water were homogenized and further [10.sup.-] fold dilutions ([10.sup.-2], [10.sup.-3]) were prepared and analyzed.
A sample of beef (10.0 g) was well mixed in 90 ml diluent (Saline peptone
containing, g/L Distilled water; 1.0 g, Peptone
; 8.5 g, NaCl; pH 7.0 [+ or -] 0.2 and autoclaved at 121 [degrees]C for 15 min.) and prepared into suitable dilutions.
These were individually swabbed with sterile cotton swabs (moistened previously with 0.1% peptone
When similar experiments were conducted in OPPSS broth, which contains proteose peptone
as an additional carbon source, degradation patterns changed for most of the isolates (Table 2).
Skin sample was placed in a sterile plastic bag with 45 ml of 0.1% peptone
buffer and pummeled in a Stomacher for 2 min.
After 20 hours of pre-enrichment in buffered peptone
water, both protocols were sensitive enough to identify all positive samples.
Starch casein agar, agar, asparagine, Luria Bertani broth, dextrose, maltose, D-(+)-galactose, soya peptone
, yeast extract powder and dialysis membranes-150 were purchased from HiMedia laboratories, India.
Culture media: Glucose, (Acros Organics), bacteriological peptone
, malt extract, yeast extract powder and nutrient agar (Lab M Limited), K2HPO4 (Laboratory Reagent), MgSO4 (Oxford Laboratory Reagent), agar (Sisco Research Laboratories Pvt.