The effect of peptones on transfection efficiency and productivity
In the next step, the effect of three different peptones on transfection efficiency and recombinant protein yield were examined.
Peptones are widely used as supplements for serum-free culture media.
Fortification of a protein-free cell culture medium with plant peptones improves cultivation and productivity of an interferon-gamma-producing CHO cell line.
Moreover, peptone feeding strategy used here was successful to increase volumetric produc-tivities up to 37[degrees]o.
Conclusion: Here we described an optimization process for TGE in suspension-adapted CHO cells based on Polyethylenimine (PED/DNA concentration, DNA: PEI ratio, starting cell densities and peptone feeding strategy.
In this study, it was proposed that peptone supplementation is a promising approach for TGE optimization.
This probably happened due to the supplementation with yeast extract and peptone containing trace elements.
Effect of adding casein and inositol, peptone, yeast extract, B-vitamins and amino acids
The addition of both peptone and yeast enabled the fastest growth.
Treatments Means (1) (mm [day.sup.-1]) BM + peptone 0.1% + yeast 0.1% 3.4562 a BM + yeast 0.1% 2.7937 ab BM + amino acids 0.01% 2.2375 bc BM + peptone 0.1% 2.0750 bc BM 1.4812 c BM + B-vitamins 0.01% 1.4187 c (1) Means followed by a different letter are significantly different at the 5% level according to Tukey*s honestly significant difference test.