In addition to its role in copper homeostasis, CueO has also been found to oxidize iron (Grass and Rensing, 2001; Kim et al., 2001) which further complicates its exact role in periplasmic space
. Outten et al.
Located in periplasmic space
flagellar proteins (traditionally denoted as Fla) are widely presented in T.
At pH 7.0, biogenic gold NPs (~10-20nm) were deposited in the periplasmic space
The SHV-104 was extracted from the periplasmic space
and purified by three chromatographic steps (Q-Sepharose FF, HiTrap Q-Sepharose HP, and molecular sieve Sephacryl-100) to obtain at the end ~ 10 mg (90% protein pure) for liter.
coli cells grown on normal medium displayed a cell wall composed of outer membrane and cytoplasmic membrane enclosing a periplasmic space
. The nucleoid was centrally located and contained fine DNA fibrils.
In Gram-negative bacilli, [beta]-lactamase enzymes remain in the periplasmic space
, where they attack the [beta]-lactam before it can reach the PBPs.
The effect of polymyxin B on release of CTLT from periplasmic space
was studied with cultures grown in GCIV medium.
Nitrocefin is normally excluded by the outer cell membrane, but if it is able to pass this barrier, then it will be cleaved by the enzyme, [beta]-lactamase, which is localized within the periplasmic space
(Angus et al.
Thus, PorB was expressed in the periplasmic space
of VCG using the vector pMALp2x, as a fusion protein of the maltose-binding protein.