At 70degC, persulphate
ions (S2O8) of initiator (APS) decompose and produce SO free radicals which initiate polymerization reaction.
A recipe using 50 g/l of hydrogen peroxide (50%) 30 g/l sodium hydroxide (Flake) 25 g/l sodium silicate 5 g/l sodium persulphate
5 g/l nonionic surfactant and 0.
Fibroblast 3T3 cell line was purchased from (NCCS, Pune), Pune, india, poly (2-Hydroxy ethyl metha acrylate), sodium meta bisulphate, potassium persulphate
, silver sulfate, sodium borohydrate, PBS 10%, Dulbecco's Modified Eagle Medium (DMEM) containing serum was purchased from Sigma-Aldrich.
This work is in continuation to our investigations pertaining to the role of Mn(II) and Co(II) as catalysts on oxidations by persulphate
The ABTS+ stock solution was prepared by reacting potassium persulphate
mM potassium persulphate
and allowing the solution to stand in dark at room temperature for 12-18 hours before use.
Ascorbic acid, 2,27-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), acrylamide, ammonium persulphate
(APS), Tris-buffered saline, and glycine were purchased from Sigma Aldrich.
Wet-chemical oxidation with persulphate
and UV light is a widely applied method, especially in pure water and ultra-pure water.
In this method, NIPAM monomers are made to react with a cross linking agent like N-N'-methylene-bis-acrylamide (BIS) along with an initiator like potassium persulphate
(KPS) in nitrogen environment.
Acrylamide, bis-acrylamide, N-N-N' -N' tetramethyl ethylenediamine, ammonium persulphate
and Tween-20 were obtained from Bio-Rad Laboratories, U.
Abstract A soluble copolymer from aniline and o-toluidine [poly (aniline-co-o-toluidine)] was synthesized by chemical oxidative copolymerization using ammonium persulphate
as an oxidant in hydrochloride aqueous medium.
Phosphorus and nitrogen contents were determined in unfiltered samples using persulphate
oxidation of all moieties of each respective element present in a water sample to phosphate nitrate according to the protocols of Menzel and Corwin (17), Murphy and Riley (18), Crumpton et al (19), and Bachman and Canfield (20).