Phase-Contrast Microscopy


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Phase-Contrast Microscopy

 

a research technique in microscopy that uses special equipment to produce high-contrast images of colorless, transparent objects with different structure densities, such as living microoganisms and tissue cultures.

References in periodicals archive ?
Lack of 100% correlation of vaginal biocoenosis test results with the cytological results according to the Bethesda system means that assessing the vaginal microflora in phase-contrast microscopy should not be abandoned.
There are several features which represent the characteristics of phase-contrast microscopy images, such as SIFT [4], GIST [5], etc.
The sensitivity of this technique can be further increased by using phase-contrast microscopy or examination at 400x magnification.
Figure 2 shows micrographs obtained by phase-contrast microscopy of cross section (a) H-SC, (b) H-Q, (c) HSCA, and (d) H-QA sheet samples.
Samples were examined under phase-contrast microscopy by using an improved Neubauer hemocytometer (Hawksley and Sons, Lancing, UK).
This reprint of the original 2003 edition covers: elementary principles of phase-contrast microscopy, electron optics, wave optics, coherence and Fourier optics, STEM and Z-contrast, electron sources and detectors, measurement of electron-optical parameters, instabilities and the microscope environment, experimental methods, and associated techniques.
Trypan blue exclusion assay, phase-contrast microscopy, loss of cell adherence, and MTT assay were used to evaluate cadmium toxicity.
The upper reference limits for phase-contrast microscopy used in our laboratory are as follows: erythrocytes, <2/high-power field (HPF); leukocytes, <4/HPF; small-sized hyaline casts, <4 in each slide; hyaline with medium or large diameter, cellular, granular, granulo-hematic, waxy casts, none; cystine crystals, none; other crystals, rare (<10 in each slide).
These fractions were free of spores and aggregates of any kind, as observed by phase-contrast microscopy, SEM, and incubation on LB agar plates.
The oocysts cannot be detected by standard ova and parasite testing; methods specific for Cyclospora include modified acid-fast or other stains, autofluorescence with ultraviolet epifluorescence microscopy, and wet mount under phase-contrast microscopy (3).
The only subtle difference is that the Mg-actin bundles tend to reach a larger size than PEG-actin bundles, and the difference is more apparent by phase-contrast microscopy (data not shown).

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