Release date- 12082019 - Mallinckrodt plc (NYSE: MNK), a global biopharmaceutical company, today confirmed enrollment of the first patient in the company's Phase 2a study assessing the pharmacokinetics, pharmacodynamics and safety of the oral investigational drug MNK-6106 (L-ornithine
phenylacetate) versus rifaximin in patients with hepatic (liver) cirrhosis and hepatic encephalopathy (HE).
The arylesterase and paraoxonase activities of PON1 were measured spectrophotometrically using the substrates
phenylacetate and paraoxon (O, O-diethyl O-(4-nitrophenyl) phosphate), respectively [23].
Arylesterase activity of paraoxonase 1 (POX-1) was measured spectrophotometrically using
phenylacetate as a substrate (28-29).
In the study, 10 patients transitioned to Raviciti from sodium phenylbutyrate, three transitioned from intravenous sodium benzoate and sodium
phenylacetate, and three were treatment naive.
The
phenylacetate technique (SigmaAldrich) was used to measure the activity ofthis enzyme.
Also, although the two enzymes have different natural substrates, the PON has the ability to hydrolyse
phenylacetate, the natural substrate of ARE.
Treatment with sodium benzoate and sodium
phenylacetate, used in urea cycle disorders, is recommended if the ammonium levels are greater than 100 uM [27].
Moreover, phenylacetylglutamine is a product that is formed by the conjugation of
phenylacetate and glutamine.
In detail, sweetness, umami, richness, and acceptance, marked with small circles, were closely located with ethyl
phenylacetate, 5-methyl furfural, amyl cinnamal, ethyl myristate, decyl aldehyde, 1-phenylethyl acetate, 1-octen-3-ol, 3-buten-2-ol, butanoic acid, and caproaldehyde on the right side along the X-matrix.
(vi) Arylesterase enzymatic activity of paraoxonase-1 (PON-1) was assayed by measuring initial hydrolysis rates of
phenylacetate as described in detail in previous reports [14,16,22].