The reaction is shown for phenylhydrazine
in Scheme 4.
Derivatization of MMDA and MDA with phenylhydrazine
yielded 4-methyl-1-phenylpyrazole and 1-phenylpyrazole, respectively.
2] was then added, and the sample derivatized with 1 [micro]mol of phenylhydrazine
for 30 min at room temperature (final volume, 0.
A well-known similar reaction takes place between some diamines, such as phenylhydrazine
, and compounds such as glucose or anhydroglucose.
Protective effect of the flavonoid silybin dihemisuccinate on the toxicity of phenylhydrazine
on rat liver.
Susceptibility of LDL to oxidation was tested by formation of thiobarbituric acid reacting substances (TBARS) after phenylhydrazine
exposure (16), and serum fatty acid profiles were determined by capillary gas chromatography (17).
radical scavenging activity by DPPH reduction, superoxide radical scavenging activity in riboflavin/light/NBT system, nitric oxide (NO) radical scavenging activity in sodium nitroprusside/Greiss reagent system and inhibition of lipid peroxidation induced by iron-ADP-ascorbate in liver homogenate and phenylhydrazine
induced haemolysis in erythrocyte membrane stabilization study.
We added hydrazine monohydrate to the reaction mixture to trap cholest-4-ene-3-one, similar to the phenylhydrazine
reaction used to produce osazone from aldose (15).
was used as prooxidant to treat fresh LDL samples in a two-step procedure: (a) LDL solutions (200 [micro]L) were incubated with 20 [micro]L of 0.
Albumin (human, fraction V), 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), nitroblue tetrazolium, sodium dodecyl sulfate (SDS), SOD (from human erythrocytes), 1,1,3,3-tetraethoxypropane, thiobarbituric acid (TBA), and xanthine were purchased from Sigma; phenylhydrazine
hydrochloride, metaphosphoric acid (MPA), phosphotungstic acid, and sodium azide were from Riedel; n-butanol, Tris, CUS[O.