The Folin-Denis reagent was prepared by mixing 100 g sodium tungstate, 20 g phosphomolybdic acid
and 50 ml phosphoric acid with 750 ml water.
USA); and phosphomolybdic acid was obtained from BDH, Dorset, UK.
An equal volume of each extract and 2% solution of phosphomolybdic acid in absolute ethanol were mixed at room temperature, and the absorbance was measured by a spectrophotometer (UNICAM UV 300) at 492 nm after 5 min (Attard and Scicluna-Spiteri, 2001).
The inorganic phosphorus concentration of serum was measured using the phosphomolybdic acid
method (Fiske and Subbarow, 1925).
Potassium hydroxide was from Chemapol, Checkoslovakia; manganese(II)chloride, sulphuric acid, and phosphomolybdic acid
were from Reakhim, Russia.
Tested for Presence/ Test performed absence * Phenols +++ Phosphomolybdic acid
test Tannins +++ Braemer's test Steroids and ++ Liebermann- Terpenoids (Terpenoids) Burchardt test Alkaloids - Dragendorff's test Anthraquinones - Borntrager test Flavonoids ++ Shinoda's test * - absent + Traces +++ Abundant Table 2 TLC finger printing profile of methanolic extract of H.