This pollen mixture
was shaked at 4[degrees]C for 24 h, and after centrifugation (4600 gr in 50 min); produced fluid was kept at 20[degrees]C until its use.
Materials * binocular microscope * student light microscope * fresh Cleome flowers (collected the morning of lab) * expanding fruit (ovary) from Cleome flowers that are two to three days post-pollination * prepared slides of a pollen mixture
(Carolina Biological) * store-bought flowers (optional) * microscope slides and cover slips * disposable pipets * small beakers of water * dissecting probes, scalpel * Petri dishes (100 mm) Activities 1.
The total extracts of honeybee-collected pollen mixture
and the six different constituent pollens were effective antioxidants as free radical scavengers, although with lower levels of antiradical activities than all the standards tested.
The mean number of seed per pod for the control was below the normal average (10-15 seeds [pod.sub.-1]) as reported by Beuselinck and Grant (1995), but this result might be an expression of incompatibility since the pollen mixture
used was derived from only three genotypes.
To test for differences in the degree of seed set reduction between subspecies, the proportion of maximum subspecies seed set was compared between subspecies for both the 5% immigrant and 50% immigrant pollen mixture
Furthermore, if there were an agronomic application of xenia, through the planting of a mixture of two hybrids, it would seem better to evaluate the advantage of cross fertilization over self fertilization with a pollen mixture
on the same plant.
They then performed a similar simulated herbivory study on 20 plants of the wild gourd except this time they examined pollen performance via a pollen mixture
Low temperature effect on selective fertilization of pollen mixtures
of wild and cultivated tomato species.
In fact, when 50:50 pollen mixtures
were applied, this prediction was borne out only for I.
Therefore, pollen mixtures
may mimic the composition of natural pollen loads.
Total 236 412 648 36:64 Other studies using pollen mixtures
have shown that differences in the proportion of seeds/seedlings sired by pollen from nutrient-stressed and nutrient-enriched plants are due to prezygotic factors (speed of germination and/or pollen tube growth rates) (Young and Stanton 1990b, Lau and Stephenson 1993, 1994).
More precise methods of controlling the pollen mixtures
(e.g., Griffin et al.