When considering the detection of infrared radiation (IR) with pure and doped Polyvinylidene difluoride
(PVDF film), using the mechanism of pyroelectricity, considerable research has been conducted.
Extracted proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride
The Hybond-P polyvinylidene difluoride
(PVDF) membrane, enhanced chemiluminescence (ECL) Western blotting detection reagent, and analysis system were obtained from Amersham (Buckinghamshire, UK).
A total of 40 [micro]g of protein in each sample was subjected to SDS-PAGE with 10% gels, followed by electrotransfer to polyvinylidene difluoride
Clarke, "Enhanced ferroelectric phase content of polyvinylidene difluoride
fibers with the addition of magnetic nanoparticles," Langmuir, vol.
The polyvinylidene difluoride
(PVDF) membranes and the antibody against [beta]-actin were purchased from Millipore Corporation (Bedford, MA, USA).
Western blot: Equivalent amount of protein obtained from medial EC loaded to each well, separated by 12 % SDS-PAGE, and transferred to the polyvinylidene difluoride
A key component of this process is ultra-filtration, which uses 0.4 p polyvinylidene difluoride
(PVIN) membranes with an upper pH limit of 10.0.
After removing the plate from the solvent mixture, the plate is mounted together with a polyvinylidene difluoride
(PVDF) membrane, a teflon sheet and glass fiber sheet.
Storsberg achieved this by selectively altering the base material, polyvinylidene difluoride
, by coating the fluoride synthetic tissue with a reactive molecule.
The proteins separated by 12% SDS-PAGE were transferred onto polyvinylidene difluoride
(PVDF) membrane (Roche, Germany) by using a semi dry blotter unit (Peqlab, Germany).
For Western blot analysis, we loaded 30 [micro]g total protein extracted from commercially available left ventricle (LV) from healthy humans (ILSbio, LLC) onto polyacrylamide gels and transferred them onto polyvinylidene difluoride