Then electro-transferred onto polyvinylidenedifluoride
Protein extracts were loaded onto a 12% sodium dodecyl sulphate- polyacrylamide gel (SDS-polyacrylamide gel) (40 [micro]g/well) and electrophoretically transferred to polyvinylidenedifluoride
For Western blot analysis, 40 [micro]l of total protein was separated on 7.5% SDS-PAGE gel and transferred to a polyvinylidenedifluoride
transfer membrane (PVDF) (Bio-Rad, Richmond, CA, USA) for 16 h at 150 mA, using transfer buffer (25 mM TrisHCl, 190 mM glycine, 20% methanol, and 0.05% SDS).
Laoui, "Monolayer graphene transfer onto polypropylene and polyvinylidenedifluoride
microfiltration membranes for water desalination," Desalination, vol.
Briefly, lysates were separated using SDS-PAGE and were electrophoretically transferred to a polyvinylidenedifluoride
Lysates (20 [micro]g protein/lane) were separated by electrophoresis on 8% SDS PAGE gels under reducing conditions, then transferred to polyvinylidenedifluoride
membranes (BioRad), using Biorad Miniprotean system (BioRad).
An equal amount of protein (25 [micro]g/lane) from each cell lysate was separated on gel electrophoresis (sodium dodecyl sulfate-polyacrylamide electrophoresis) and electrophoretically transferred to polyvinylidenedifluoride
membranes (Immobilon, Millipore, Billerica, MA, USA).
Protein bands were transferred to polyvinylidenedifluoride
membranes and blocked with 5% non-fat milk for 1 h.
Equivalent amounts of total protein (60 [micro]g) were separated by 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidenedifluoride
membranes (Millipore Co., Billerica, MA, USA).
Equal amounts of protein from each sample were separated using sodium dodecyl sulfate (SDS)-10% polyacrylamide gel electrophoresis (PAGE) and transblotted onto polyvinylidenedifluoride
(PVDF) membranes (Millipore, Bedford, MA, USA).
(PVDF) membranes were purchased from Millipore (Billerica, MA, USA).
Equal amounts of protein (50 [micro]g per sample) were resolved in 10% sodium dodecyl sulfate- (SDS-) polyacrylamide gels and then transferred to nitrocellulose immobilon-P polyvinylidenedifluoride