Drabkins reagent, potassium ferricyanide, TDP, thiamin monophosphate (TMP), thiamin, and bovine serum albumin were obtained from Sigma Chemical Company.
For postcolumn derivatization of TDP, the column effluent was reacted with 2 mol/L sodium hydroxide containing 600 mg/L potassium ferricyanide at a flow rate of 0.
The remainder of the diluted hemolysate was used for hemoglobin (Hb) estimation using Drabkins reagent (Sigma Diagnostics), which contains potassium ferricyanide and potassium cyanide.
5 mL of 20% (w/v) TCA and then made up to total volume of 10 mL with DW following incubation with 2 mL of 5 mM potassium ferricyanide
in an ice under the dark for 60 min.
5ml of 1% potassium ferricyanide
were added and incubated for 20minutes at 50 [degrees]C.
Analytically pure sodium hydroxide (NaOH) and potassium ferricyanide
were supplied by Xiaoshan Chemistry Co.
To assess the stability of the thiochrome derivatives, controls and patients samples (n = 25) were assayed immediately after potassium ferricyanide
oxidation, then stored at 10[degrees]C in the dark and reassayed after 24 and 48 h.
After cleaning and drying the cup and measuring spoons, mix 1 cup hot water with 2-1/4 tablespoons potassium ferricyanide.
He mixes 1 pound ferric ammonium citrate with 1 gallon hot tap water, stirs until crystals are dissolved, then adds 1/2 pound potassium ferricyanide.
Reagent 4 contained 15 mmol/L potassium ferricyanide
in 20 mmol/L potassium phosphate (pH 7.
The enzymatic reaction was stopped by addition of 50 [micro]L of chromophore reagent (40 mmol/L DBPDA in 6 mol/L HCI), followed by addition of 50 [micro]L of oxidizing agent (40 mmol/L potassium ferricyanide
in 20 mmol/L potassium phosphate buffer, pH 8.
4]), followed by 30 [micro]L of oxidizing agent [5 mmol/L potassium ferricyanide
in 10 mmol/L sodium phosphate buffer (pH 7.