Three membrane pore sizes, 0.10, 0.45, and 5.00 [micro]m, were tested on this platform; 80 [micro]L of 1.2% potassium ferricyanide
in deionized water was used as the diffusion solution.
Five milliliters of homogenates were mixed with 100 [micro]L of 0.04 M ammonium sulfamate, 200 [micro]L of 0.5 M lead acetate, and 2.5 mL of 20% (w/v) TCA and then made up to total volume of 10 mL with DW following incubation with 2 mL of 5 mM potassium ferricyanide
in an ice under the dark for 60 min.
Potassium carbonate and potassium ferricyanide
were used as a secondary oxidant in tert-butyl alcohol-water mixture (1: 1).
The reducing power of yerba mate tea was examined using a mixture of 1mL of phosphate buffer (0.2 M, pH 6.6), 1mL of potassium ferricyanide
(1% by weight), and 1 mL of tested sample .
Figure 6 shows the cyclic voltammogram behaviour of polyaniline and various NDC electrodes in 0.01 M potassium ferricyanide
and hexaammineruthenium (III) in 0.1 M potassium nitrate solution.
The each sample amounting to about 0.5 ml was added to 2.5 ml 0.2 M phosphate buffer (pH 6.6) and 2.5 ml potassium ferricyanide
To each tube 1ml of methanol, 2.5ml of phosphate buffer and 2.5ml of 1% potassium ferricyanide
were added and incubated for 20minutes at 50 [degrees]C.
Analytically pure sodium hydroxide (NaOH) and potassium ferricyanide
were supplied by Xiaoshan Chemistry Co.
Before injection onto the column, samples were derivatized by addition of 50 [micro]L of freshly prepared potassium ferricyanide
in 15% sodium hydroxide (Fig.
The other chamber, the cathode, was filled with potassium ferricyanide
, a chemical that acts as an oxidizing agent to round out the electrical circuit.
1, 1-Diphenyl-2-picrylhydrazyl (DPPH), deoxyribose, thiobarbituric acid (TBA), trichioroacetic acid (TCA), Fe[Cl.sub.2], Fe[Cl.sub.3], potassium ferricyanide
([K.sub.3]Fe[CN.sub.6]), and Folin-Ciocalteu reagent were purchased from Sigma Chemical (St.
The third is the standard version of Murakami's reagent (10g potassium hydroxide, 10g potassium ferricyanide
and 100 mL distilled water) used at 122F (50c) for 3 min to color iron phosphide dark yellow or brown and leave the cementite and ferrite colorless.