The "ripe" stage in triploids, which represented the observed culmination in gonad development for triploids in this study, consisted of gonads with mostly primary spermatocytes
in males and typically few oocytes in females.
The spermatogonia and primary spermatocytes
detected in the STs, which contained MNGCs, expressed more bax protein when compared with other cells.
In Johnson score 4, the CDC25A protein is widely expressed in primary spermatocytes
. The decrease in CDC25A mRNA expression indicates an interruption in the spermatogenetic process.
The presence of primary spermatocytes
at two different stages, specifically zygotene and diplotene, with large nuclei observed in spermatocytes in the diplotene stage, was the other notable characteristic of this stage.
In our study, histological changes in diabetic control rats revealed degenerated and atrophied seminiferous tubules deficient of spermatogenic cells such as spermatogonia, primary spermatocyte
, spermatid and spermatozoa (Fig.
g: spermatogonia, R: round spermatid, S: sperms, L: lumen, and P: primary spermatocytes
; the (ES) elongated spermatids, Leydig cells in the interstitial tissue ([up arrow]) (x1000).
In a study, administration of 200 and 300 mg/kg to rats for 60 days showed a significant increase in the number of sperms, sertoli cells, and primary spermatocyte
compared to the control group.
Also it was noted in that study the emergence of large gaps (vacuolization) between spermatocytes and this result consistent with what he observed on the vacuolization in the areas between spermatogonia cells and primary spermatocytes
or between spermatogonia cells and Sertoli cells, and the cause was in the degeneration and detachment processes which occur in the spermatocytes as a result of drug treatment, and common cold was noted as well in the inflammatory infiltration with the emergence of a rupture and congestion of blood vessels [18; 32; 33].
At 4-5 years there is rise in urinary LH which is associated with Adult dark sperm cell in primary spermatocytes
(Onset of meiosis).
In males, FSH induces Sertoli cells to secrete androgen-binding proteins as well as stimulate primary spermatocytes
to undergo the first division of meiosis.
Regarding ERs, in all mouse germ cells only ER[beta] is detected [6, 17]; in the human some authors reported a complete absence of ER[alpha] [18, 19] while some others suggested its expression in primary spermatocytes
and spermatids and in the ejaculated sperm [4, 20-22].
A large number of primary spermatocytes
is always visible, which lie in the cell layer luminal to the spermatogonia.